Team:EPF Lausanne/Calendar/15 September 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Analysis of the sequencing resutls of the three promoters (sensing-module)''' <BR> The sequencing results were rather promising exce...")
Line 10: Line 10:
 +
'''MinPrep of the hya-plasmid''' <BR>
 +
Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 20:10, 30 September 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Analysis of the sequencing resutls of the three promoters (sensing-module)
The sequencing results were rather promising except for the Hya promoter. The sample that I send did not contain it. So I purified one in order to send it again on Tuesday. This sample was from one of the assays. I send this sample because it did not turn green when I added arabinose indicating that the Arabinose inducible promoter had effectively been removed and hopefully replaced by the hya promoter.

Functional assays for the three promoters (sensing-module)
I again tried to grow the bacteria in an LB medium to which I added different amounts of either 10X MOPS or 10X HEPES buffer. But again the cultures did not turn green.


MinPrep of the hya-plasmid
Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/15_September_2013"