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Team:IIT Madras/Notebook
From 2013.igem.org
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=== Experimental results === | === Experimental results === | ||
| - | === | + | === Protocol === |
| - | 1. Transformation: | + | '''1. Transformation:''' |
---- | ---- | ||
(a) Added 1-2µl of DNA to competent cell. | (a) Added 1-2µl of DNA to competent cell. | ||
| - | + | <br>(b) Kept on ice for 30 minutes. | |
| - | + | <br>(c) Heat Shock was given at 42°C for 90 sec. | |
| - | + | <br>(d) Kept on ice for 5 min. | |
| + | <br>(e) Added 400µl of LB media. | ||
| + | <br>(f) Incubated at 37°C for 1h in shaker. | ||
| + | <br>(g) Plating was done with 250µl of culture. | ||
| + | <br>(h) Plates were incubated overnight at 37°C. | ||
== Phase III == | == Phase III == | ||
Revision as of 18:55, 6 July 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Phase I : Ideation
Phase II : Experimentation
e-Notebook
| May 13 | June 13 | July 13 | ||||||||||||||||||||||||||||||||
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Experimental results
Protocol
1. Transformation:
(a) Added 1-2µl of DNA to competent cell.
(b) Kept on ice for 30 minutes.
(c) Heat Shock was given at 42°C for 90 sec.
(d) Kept on ice for 5 min.
(e) Added 400µl of LB media.
(f) Incubated at 37°C for 1h in shaker.
(g) Plating was done with 250µl of culture.
(h) Plates were incubated overnight at 37°C.
Phase III
Troubleshooting
Discussions
This page is under construction. For more information contact Rohan or Aman.
