Sophierivara (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}") |
Sandelisa90 (Talk | contribs) |
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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | '''Cell surface Display''' | ||
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| + | ''Primers arrived:'' we did a PCR to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | ||
| + | We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Primers arrived: we did a PCR to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
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