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Team:KU Leuven/Project/Glucosemodel/qPCR
From 2013.igem.org
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After preparing three samples we started with the isolation of RNA. We then used the Nanodrop to get to know the concentration of our RNA and got the following results:</p> | After preparing three samples we started with the isolation of RNA. We then used the Nanodrop to get to know the concentration of our RNA and got the following results:</p> | ||
| + | <p> | ||
<table border="1"> | <table border="1"> | ||
<tr><th></th><th>ng/µl</th><th>260/280</th><th>260/230</th></tr> | <tr><th></th><th>ng/µl</th><th>260/280</th><th>260/230</th></tr> | ||
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<tr><td>Sample C</td><td>92</td><td>2,17</td><td>1,28</td></tr> | <tr><td>Sample C</td><td>92</td><td>2,17</td><td>1,28</td></tr> | ||
</table> | </table> | ||
| + | </p> | ||
<p align="justify>These results are satisfactory. Only the 260/230 values are lower than expected, but after purification these values will rise.<br/> | <p align="justify>These results are satisfactory. Only the 260/230 values are lower than expected, but after purification these values will rise.<br/> | ||
Revision as of 19:08, 1 October 2013
Secret garden
Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!
- A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
- For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
- We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?
Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!
We perform this qPCR for two main reasons:
- With a qPCR we can check if our genes of interest are properly transcribed. This is a good characterisation of the methyl salicylate brick (BBa_K1060003)./li>
- We would like to know the amount of transcripts as an input for our methylsalicylate model.
We first started with the sample preparation, as described in the methyl salicylate brick and used 3 biological repeats.
After preparing three samples we started with the isolation of RNA. We then used the Nanodrop to get to know the concentration of our RNA and got the following results:
| ng/µl | 260/280 | 260/230 | |
|---|---|---|---|
| Sample A | 100,3 | 2,19 | 1,42 |
| Sample B | 56,3 | 2,17 | 0,78 |
| Sample C | 92 | 2,17 | 1,28 |
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