Exeter/5 August 2013

(Difference between revisions)

Revision as of 20:00, 1 October 2013

Exeter iGEM 2013 · Paint by Coli

We made up 3 replicates of:

K592018

S05058

B0015

Culture	NanoDrop concentration (ng/ul)	size
K592018	160.7	2526bp
S05058	118.3	717bp
RFP	96.1	1047bp
B0015	76.3	129bp

Part	DNA (ul)	dH20 (ul)	Buffer (ul)	BSA (ul)	Enzyme 1 (ul)	Enzyme 2 (ul)	Enzyme 3 (ul)
K592018	1.6	14.4	2.5	0.5	EcoRI 0.5	SpeI 0.5	-
S05058	2.1	13.9	2.5	0.5	EcoRI 0.5	SpeI 0.5	-
B0015	3.3	12.7	2.5	0.5	XbaI 0.5	PstI 0.5	-
AMP plasmid	10	6	2.5	0.5	EcoRI 0.5	PstI 0.5	Dpnl 0.5
RFP	2.6	13.4	2.5	0.5	EcoRI 0.5	PstI 0.5	-

Two methods using 'standard' amounts of each digest and using equimolar amounts.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli

@@ Line 39: / Line 39: @@
 ! Part !! DNA (ul) !! dH20 (ul) !! Buffer (ul) !! BSA (ul) !! Enzyme 1 (ul) !! Enzyme 2 (ul) !! Enzyme 3 (ul)
 |-
-| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
+| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
 |-
-| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
+| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
 |-
-| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || Xbal 0.5 || Pstl 0.5 || -
+| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || <i>XbaI</i> 0.5 || <i>PstI</i> 0.5 || -
 |-
-| AMP plasmid || 10 || 6 || 2.5 || 0.5 || EcoR1 0.5 || Pst1 0.5 || Dpnl 0.5
+| AMP plasmid || 10 || 6 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || Dpnl 0.5
 |-
-| RFP || 2.6 || 13.4 || 2.5 || 0.5 || EcoR1 0.5 || Pstl 0.5 || -
+| RFP || 2.6 || 13.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || -
 |}
 === Ligation ===