Team:Braunschweig/Notebook

From 2013.igem.org

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<b>Investigators: Kevin, Melanie </b><br>
<b>Investigators: Kevin, Melanie </b><br>
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Unfortunately, the agar plates from yesterday’s mixed culture didn’t show any colour which might be due to contamination. Therefore we did not evaluate these plates any further.  We also had some problems with the shaker and lost the culture containing the aeBlue construct. A test was run on how to dilute a mixed culture of the remaining eforRed and amilGFP constructs in order to be able to count colonies of each colour on large agar plates.<br>
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The transformation of the eforRed construct did not work (no colonies on agar plate) and has to be repeated.<br>
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The first continuous cultivations were carried out in order to get to know the routines and problems of a continuous cultivation. Due to problems with the regulation of pumps no valuable results were produced but the setting was improved based on these experiences.<br></p>
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<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Wednesday, August 14, 2013</p>
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<b>Investigators: Anna, Kevin </b><br>
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Transformation of the eforRed expression cassette in <i>E. coli</i> Top10 was performed by electroporation.<br>
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The reporter strains for the Las and Rhl systems (<i>E. coli</i> JM109 pSB1075 and <i>E. coli JM109 pSB406 respectively) arrived today. Liquid cultures in 2xYT containing ampicillin were inoculated and grown at 37°C and 250 rpm overnight.<br>
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Pre-cultures of <i>E. coli</i> Top10F’ containing finale pRhl inducible construct and E. coli XL1 containing finale pLas inducible construct in 50 ml 2xYT containing chloramphenicol for continuous cultures were grown at 37°C and 250 rpm overnight.</p>
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<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Thursday, August 15, 2013</p>
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<b>Investigators: Laura, Kerstin, Roman, Kevin, Jan </b><br>
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Revision as of 21:07, 1 October 2013

Labjournal

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This is the documentation of our lab work. An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.

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