Exeter/5 August 2013

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=== Glycerol stocks ===
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=== Glycerol stocks === __NOTOC__
We made up 3 replicates of:
We made up 3 replicates of:
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K592018
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[http://parts.igem.org/Part:BBa_K592018 K592018]
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S05058
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[http://parts.igem.org/Part:BBa_S05058 S05058]
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B0015
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[http://parts.igem.org/Part:BBa_B0015 B0015]
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! Part !! DNA (ul) !! dH20 (ul) !! Buffer (ul) !! BSA (ul) !! Enzyme 1 (ul) !! Enzyme 2 (ul) !! Enzyme 3 (ul)
! Part !! DNA (ul) !! dH20 (ul) !! Buffer (ul) !! BSA (ul) !! Enzyme 1 (ul) !! Enzyme 2 (ul) !! Enzyme 3 (ul)
|-
|-
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| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
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| K592018 || 1.6 || 14.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
|-
|-
-
| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || EcoR1 0.5 || Spe1 0.5 || -
+
| S05058 || 2.1 || 13.9 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>SpeI</i> 0.5 || -
|-
|-
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| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || Xbal 0.5 || Pstl 0.5 || -
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| B0015 || 3.3 || 12.7 || 2.5 || 0.5 || <i>XbaI</i> 0.5 || <i>PstI</i> 0.5 || -
|-
|-
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| AMP plasmid || 10 || 6 || 2.5 || 0.5 || EcoR1 0.5 || Pst1 0.5 || Dpnl 0.5
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| AMP plasmid || 10 || 6 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || Dpnl 0.5
|-
|-
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| RFP || 2.6 || 13.4 || 2.5 || 0.5 || EcoR1 0.5 || Pstl 0.5 || -  
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| RFP || 2.6 || 13.4 || 2.5 || 0.5 || <i>EcoRI</i> 0.5 || <i>PstI</i> 0.5 || -  
|}
|}
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=== Ligation ===
=== Ligation ===

Latest revision as of 19:49, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Glycerol stocks

We made up 3 replicates of:

[http://parts.igem.org/Part:BBa_K592018 K592018]

[http://parts.igem.org/Part:BBa_S05058 S05058]

[http://parts.igem.org/Part:BBa_B0015 B0015]


Nano Drop

Culture NanoDrop concentration (ng/ul) size
K592018 160.7 2526bp
S05058 118.3 717bp
RFP 96.1 1047bp
B0015 76.3 129bp


Digestion

Part DNA (ul) dH20 (ul) Buffer (ul) BSA (ul) Enzyme 1 (ul) Enzyme 2 (ul) Enzyme 3 (ul)
K592018 1.6 14.4 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
S05058 2.1 13.9 2.5 0.5 EcoRI 0.5 SpeI 0.5 -
B0015 3.3 12.7 2.5 0.5 XbaI 0.5 PstI 0.5 -
AMP plasmid 10 6 2.5 0.5 EcoRI 0.5 PstI 0.5 Dpnl 0.5
RFP 2.6 13.4 2.5 0.5 EcoRI 0.5 PstI 0.5 -

Ligation

Two methods using 'standard' amounts of each digest and using equimolar amounts.

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/5_August_2013"