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''Primers arrived:'' we did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | ''Primers arrived:'' we did a PCR (see protocols) to amplifay ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. | ||
We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
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| + | '''General''' | ||
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| + | Competent cells try 2. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Primers arrived: we did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. We thus dis a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
