Exeter/3 September 2013

From 2013.igem.org

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== Results of liquid cultures from yesterday ==
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{{:Team:Exeter/Template/Header}}
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== Results of liquid cultures from yesterday == __NOTOC__
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{| class="wikitable"
{| class="wikitable"
|-
|-
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! Part !! Did it work? !! Source
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! Part !! Did it work?
|-
|-
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| NP1 (CcaS + Terminator) || ✗ || Transformation plate
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| <i>ompF</i> + RBS A || ✓
|-
|-
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| NP2 (promoter, RBS, FixJ + Terminator || ✗ || Transformation plate
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| <i>ompF</i> + RBS B || ✓ 
|-
|-
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| NP3 (yellow pigment + Terminator) || ✗ || Transformation plate
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| <i>ompF</i> + RBS C || ✓ 
|-
|-
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| NP4 (magenta pigment + Terminator) || ✗ || Transformation plate
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| <i>ompF</i> + RBS D || ✓ 
|-
|-
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| NP7 (YF1 + Terminator) || ✓ || Transformation plate
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| K592022 A || ✗ 
|-
|-
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| NP8 (FixJ + Terminator) || ✓ || Transformation plate
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| K592022 B || ✓
|-
|-
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| Ligation control || ✗ || Transformation plate
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| K592011 A|| ✗
|-
|-
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| Lambda inverter system || ✗ || Streak plate in fridge
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| K592011 B || ✓ 
|-
|-
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| T7 promoter || ✗ || Streak plate in fridge
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| K864404 || ✓ 
|-
|-
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| Promoter + RBS (BBa_K608002) || ✓ || Streak plate in fridge
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|}
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== Miniprepped ==
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*<i>ompC</i> + RBS A
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*<i>ompC</i> + RBS B
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*<i>ompC</i> + RBS C
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*<i>ompC</i> + RBS D
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*[http://parts.igem.org/Part:BBa_K592011 K592011 B]
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*gblocks 1 to 15
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== NanoDrop from miniprep ==
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{| class="wikitable"
|-
|-
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| RBS (BBa_B0034) || ✓ || Streak plate in fridge
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! Part !! Concentration (ng/ul)
|-
|-
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| OmpR "A" || ✓ || Streak plate in fridge
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| gBlock 1 || 27.4
|-
|-
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| OmpR "B" || ✗ || Streak plate in fridge
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| gBlock 2 || 28.6
|-
|-
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| RFP control || ✓ || Transformation plate
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| gBlock 3 || 101.2
|-
|-
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| RBS + cph8 (BBa_K592018) || ✓ || Transformation plate
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| gBlock 4 || 37.2
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|-
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| gBlock 5 || 168.6
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|-
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| gBlock 6 || 21.3
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|-
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| gBlock 7 || 153.7
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|-
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| gBlock 8 || 30.8
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|-
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| gBlock 9 || 247.1
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|-
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| gBlock 10 || 183.4
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|-
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| gBlock 11 || 197.4
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|-
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| gBlock 12 || 105.1
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|-
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| gBlock 13 || 126.6
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|-
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| gBlock 14 || 172.5
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|-
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| gBlock 15 || 87.0
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|-
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| <i>ompF</i> + RBS A  || 41.2
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|-
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| <i>ompF</i> + RBS B  || 57.8
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|-
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| <i>ompF</i> + RBS C  || 74.2
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|-
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| <i>ompF</i> + RBS D  || 46.7
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|-
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| K592011 B  || 159.0
|}
|}
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Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook].
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  </div>
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</div>
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{{:Team:Exeter/Template/Footer}}

Latest revision as of 20:45, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Results of liquid cultures from yesterday

gBlocks

colours of our liquid cultures
gBlock Colour
1 No
2 No
3 Vibrant pink
4 No
5 Vibrant pink
6 No
7 Peach
8 No
9 Pink - not so vibrant
10 Vibrant pink
11 Vibrant pink
12 Pink - not so vibrant
13 Pink - not so vibrant
14 No
15 No


Part Did it work?
ompF + RBS A ✓
ompF + RBS B ✓
ompF + RBS C ✓
ompF + RBS D ✓
K592022 A ✗
K592022 B ✓
K592011 A ✗
K592011 B ✓
K864404 ✓


Miniprepped

  • ompC + RBS A
  • ompC + RBS B
  • ompC + RBS C
  • ompC + RBS D
  • [http://parts.igem.org/Part:BBa_K592011 K592011 B]
  • gblocks 1 to 15

NanoDrop from miniprep

Part Concentration (ng/ul)
gBlock 1 27.4
gBlock 2 28.6
gBlock 3 101.2
gBlock 4 37.2
gBlock 5 168.6
gBlock 6 21.3
gBlock 7 153.7
gBlock 8 30.8
gBlock 9 247.1
gBlock 10 183.4
gBlock 11 197.4
gBlock 12 105.1
gBlock 13 126.6
gBlock 14 172.5
gBlock 15 87.0
ompF + RBS A 41.2
ompF + RBS B 57.8
ompF + RBS C 74.2
ompF + RBS D 46.7
K592011 B 159.0

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/3_September_2013"