Exeter/3 September 2013

From 2013.igem.org

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         <div class="span" style="text-align:justify">
         <div class="span" style="text-align:justify">
-
== Results of liquid cultures from yesterday ==
+
== Results of liquid cultures from yesterday == __NOTOC__
Line 51: Line 51:
! Part !! Did it work?   
! Part !! Did it work?   
|-
|-
-
| OmpF + RBS A || ✓  
+
| <i>ompF</i> + RBS A || ✓  
|-
|-
-
| OmpF + RBS B || ✓   
+
| <i>ompF</i> + RBS B || ✓   
|-
|-
-
| OmpF + RBS C || ✓   
+
| <i>ompF</i> + RBS C || ✓   
|-
|-
-
| OmpF + RBS D || ✓   
+
| <i>ompF</i> + RBS D || ✓   
|-
|-
| K592022 A || ✗   
| K592022 A || ✗   
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== Miniprepped ==
== Miniprepped ==
-
*OmpF + RBS A
+
*<i>ompC</i> + RBS A
-
*OmpF + RBS B
+
*<i>ompC</i> + RBS B
-
*OmpF + RBS C
+
*<i>ompC</i> + RBS C
-
*OmpF + RBS D
+
*<i>ompC</i> + RBS D
-
*K592011 B  
+
*[http://parts.igem.org/Part:BBa_K592011 K592011 B]
*gblocks 1 to 15
*gblocks 1 to 15
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| gBlock 15 || 87.0
| gBlock 15 || 87.0
|-
|-
-
| OmpF + RBS A  || 41.2
+
| <i>ompF</i> + RBS A  || 41.2
|-
|-
-
| OmpF + RBS B  || 57.8
+
| <i>ompF</i> + RBS B  || 57.8
|-
|-
-
| OmpF + RBS C  || 74.2
+
| <i>ompF</i> + RBS C  || 74.2
|-
|-
-
| OmpF + RBS D  || 46.7
+
| <i>ompF</i> + RBS D  || 46.7
|-
|-
| K592011 B  || 159.0
| K592011 B  || 159.0

Latest revision as of 20:45, 2 October 2013

Exeter iGEM 2013 · Paint by Coli

Results of liquid cultures from yesterday

gBlocks

colours of our liquid cultures
gBlock Colour
1 No
2 No
3 Vibrant pink
4 No
5 Vibrant pink
6 No
7 Peach
8 No
9 Pink - not so vibrant
10 Vibrant pink
11 Vibrant pink
12 Pink - not so vibrant
13 Pink - not so vibrant
14 No
15 No


Part Did it work?
ompF + RBS A ✓
ompF + RBS B ✓
ompF + RBS C ✓
ompF + RBS D ✓
K592022 A ✗
K592022 B ✓
K592011 A ✗
K592011 B ✓
K864404 ✓


Miniprepped

  • ompC + RBS A
  • ompC + RBS B
  • ompC + RBS C
  • ompC + RBS D
  • [http://parts.igem.org/Part:BBa_K592011 K592011 B]
  • gblocks 1 to 15

NanoDrop from miniprep

Part Concentration (ng/ul)
gBlock 1 27.4
gBlock 2 28.6
gBlock 3 101.2
gBlock 4 37.2
gBlock 5 168.6
gBlock 6 21.3
gBlock 7 153.7
gBlock 8 30.8
gBlock 9 247.1
gBlock 10 183.4
gBlock 11 197.4
gBlock 12 105.1
gBlock 13 126.6
gBlock 14 172.5
gBlock 15 87.0
ompF + RBS A 41.2
ompF + RBS B 57.8
ompF + RBS C 74.2
ompF + RBS D 46.7
K592011 B 159.0

Take me back to the notebook.

Exeter iGEM 2013 · Paint by Coli
Retrieved from "http://2013.igem.org/Exeter/3_September_2013"