Team:UNITN-Trento/Notebook/Labposts/06/32

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(Created page with "{ "date" : "2013-06-14", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters (part 1)!!", "content" : "<html>First, we extracted K090504 and K090501 (gram+ consi...")
 
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{
{
-
"date" : "2013-06-14",
+
"date" : "2013-06-17",
"author" : "fabio-bruno",
"author" : "fabio-bruno",
-
"title" : "bacillus subtilis promoters (part 1)!!",
+
"title" : "bacillus subtilis promoters (part2)!!",
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"content" : "<html>First, we extracted K090504 and K090501 (gram+ consitutive promoter and gram+ IPTG inducible promoter)from 2012 kit n5. Then we tried to transform 2 ul of them in 100 ul of NEB10B cells. Furthermore we transformed 3 ul of K823000, K823002, K823003, K143012 in 200 ul of NEB10B. </html>",
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"content" : "<html> with some tips we selected 2 colonies for each promoter and then put them in LB ( 10 ml) for an overnight incubation (under shake). In the meanwhile we received a new e.coli strain ( NEB5a) and made an inoculum overnight. </html>",
"tags" : "Pveg-PliaG-PlepA"
"tags" : "Pveg-PliaG-PlepA"
}
}

Latest revision as of 07:48, 3 October 2013

{ "date" : "2013-06-17", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters (part2)!!", "content" : " with some tips we selected 2 colonies for each promoter and then put them in LB ( 10 ml) for an overnight incubation (under shake). In the meanwhile we received a new e.coli strain ( NEB5a) and made an inoculum overnight. ", "tags" : "Pveg-PliaG-PlepA" }

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