Team:UNITN-Trento/Notebook/Labposts/06/43

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(Difference between revisions)
 
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{
{
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"date" : "2013-06-22",
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"date" : "2013-06-20",
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"author" : "bruno",
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"author" : "emil",
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"title" : "Minipreps and screening of Promoters",
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"title" : "Linearization of psB1C3 (Failed)",
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"content" : "<html>From inocula of the day before I made the minipreps by the following<a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">protocol</a>.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>ng/ul</th></tr><tr><td>K090501</td><td>117,1</td></tr><tr><td>K090504_a</td><td>115,3</td></tr><tr><td>K090504_b</td><td>101,3</td></tr><tr><td>K823000_a</td><td>156,0</td></tr><tr><td>K823000_b</td><td>171,1</td></tr><tr><td>K823000</td><td>115,5</td></tr></table></center></html>}}<html>I made the screening test. To do that we digested 1500 ng of DNA with EcorI and PstI following <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">the screening digestion protocol</a>. <br>I used a 1.5% concentration of agarose to create a gel and I used Ethidium bromide instead the normal EuroSafe. In addition I have not used a normal Dye with the 20ul of sample loaded but I used 4ul of 30% glycerol. </html>",
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"content" : "<html>I have re-tried to linearize through a PCR the backbone PsB1C3 that we have previously purified and quntified.<br>I have tried to amplify the sample of 117.3 ng/&micro;l, 105.7 ng/&micro;l and  one of Girelli's sample(184.1 ng/&micro;l)following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols\">Phusion PCR protocol</a> and the universal primers.<br>No one of this PCRs has succeded!  </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Linearization attempt 2|<html><center>width=\"450px\" /></html>}}<html></html>",
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"tags" : "lacOid-PliaG-Pgram+"
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"tags" : "pSB1C3"
}
}

Latest revision as of 07:51, 3 October 2013

{ "date" : "2013-06-20", "author" : "emil", "title" : "Linearization of psB1C3 (Failed)", "content" : "I have re-tried to linearize through a PCR the backbone PsB1C3 that we have previously purified and quntified.
I have tried to amplify the sample of 117.3 ng/µl, 105.7 ng/µl and one of Girelli's sample(184.1 ng/µl)following the Phusion PCR protocol and the universal primers.
No one of this PCRs has succeded!

Linearization attempt 2
width=\"450px\" />
", "tags" : "pSB1C3" }

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