Team:UNITN-Trento/Notebook/Labposts/06/62

From 2013.igem.org

(Difference between revisions)

Latest revision as of 07:58, 3 October 2013

{ "date" : "2013-06-27", "author" : "emil", "title" : "Transformation of 2 GFPs and inocula of B. subtilis backbones", "content" : "Emil transformed two construct of the ancient Igem competition: both consist in a RBS with the GFP(E0040) and 2 terminators(B0010)(B0012) and are contained in bSB1A2

GFP

ID	Quantification	RBS strength
BBa_E0240	108.5 ng/µl	medium
BBa_E0840	129 ng/µl	strong

Emil followed the transformation protocol loading 150 ng of each sample.Then Emil plated the product on an Ampicillin plate.
Moreover Emil did the inocula (in LB + Ampicillin) of two backbones(BBa_823024 and BBa_823026) from the plates of Fabio and Bruno(2 inocula for each sample).", "tags" : "BBa_823024-BBa_823026-BBa_E0240-BBa_E0840-B. subtilis" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-21",
+	"date" : "2013-06-27",
-	"author" : "fabio-bruno",
+	"author" : "emil",
-	"title" : "bacillus subtilis promoters and  plasmids (part 6)!!",
+	"title" : "Transformation of 2 GFPs and inocula of B. subtilis backbones",
-	"content" : "<html> The last battle!!! Today we screened successfully three parts, K823003, K823002, K143012 . We used a brand new formula: first of all we digested 1500 ng of DNA in 50 ul. Then we changed the gel composition (1,5 % agarose, 3,5 ul of etidium bromide in 35 ml of gel). Finally we loaded the 100 bp marker along with the 1000kb marker and the parts with a loading dye missing of the dye ( 30% glicerole solution instead). We put this unusual gel in the electrophoresis chamber for 20 minutes. We obtained dim but still present traces.In the afternoon we extracted a new Bacillus promoter (PliaI, K823001) from 2013 registry kit (plate 1 20c) and transformed both in 100 ul of NEB10b and NEB5a.We also did the inocula for the parts plated yesterday.</html>",
+	"content" : "<html>Emil transformed two construct of the ancient Igem competition: both consist in a RBS with the GFP(E0040) and 2 terminators(B0010)(B0012) and are contained in bSB1A2</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|GFP|<html><center><table class=\"tn-sp-table\"><tr><th>ID</th><th>Quantification</th><th>RBS strength</th></tr><tr><td>BBa_E0240</td><td>108.5 ng/&micro;l</td><td>medium</td></tr><tr><td>BBa_E0840</td><td>129 ng/&micro;l</td><td>strong</td></tr></table></center></html>}}<html>Emil followed the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\">transformation protocol</a> loading 150 ng of each sample.Then Emil plated the product on an Ampicillin plate.<br>Moreover Emil did the inocula (in LB + Ampicillin) of two backbones(BBa_823024 and BBa_823026) from the plates of Fabio and Bruno(2 inocula for each sample).</html>",
-	"tags" : " Pveg-PliaG-PlepA-plasmidPxil-plasmidPspac-Plial"
+	"tags" : "BBa_823024-BBa_823026-BBa_E0240-BBa_E0840-B. subtilis"
 }