Team:UNITN-Trento/Notebook/Labposts/06/67
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{ | { | ||
- | "date" : "2013-06- | + | "date" : "2013-06-27", |
- | "author" : " | + | "author" : "gabriele-emil", |
- | "title" : " | + | "title" : "SAMsynthetase and R0010, the neverending story", |
- | "content" : "<html> | + | "content" : "<html>First of all Gabriele quantified the four pSB1C3+R0010 inocula of yesterday.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantity (ng/µl)</th></tr><tr><td>#A</td><td>243.7</td></tr><tr><td>#B</td><td>194.1</td></tr><tr><td>#C</td><td>245.3</td></tr><tr><td>#D</td><td>252.9</td></tr></table></center></html>}}<html><br/>Then, the four quantified samples were screened after a digestion with ExoRI-HF and PstI-HF, with the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">usual screening protocol</a> (incubated only for 45min, since both the enzymes are HF).<br/><br/>The digestion products were then run on a gel with a transparent loading dye (30% glycerol): each loaded sample contained 16µl of the sample and 4µl of transparent loading dye. Both a 1kb normal ladder and a 100bp transparent ladder were loaded, also the first well was loaded with the usual loading dye.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th>Loading scheme</th></tr><tr><td><i>Loading dye</i></td></tr><tr><td><i>Empty</i></td></tr><tr><td>100bp ladder</td></tr><tr><td>#A</td></tr><tr><td>#B</td></tr><tr><td>#C</td></tr><tr><td>#D</td></tr><tr><td><i>Empty</i></td></tr><tr><td><i>Empty</i></td></tr><tr><td>1kb ladder</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/6/62/Tn-20130627-GG_R0010pSB1C3_FAILED.jpg\" width=\"450px\" /><br/><img src=\"https://static.igem.org/mediawiki/2013/0/0b/Tn-20130627-GG_R0010pSB1C3_BIS.JPG\" height=\"450px\" /></center></html>}}<html><br/>Finally, Emil prepared the inocula of the product of ligation of R0010 and SAMsynthetase (in pSB1A2: 6 inocula of the 1:1 ligation product and 3 of the 1:3 ligation producta).</html>", |
- | "tags" : " | + | "tags" : "SAMsynthetase-Plac" |
} | } |
Latest revision as of 08:00, 3 October 2013
{ "date" : "2013-06-27", "author" : "gabriele-emil", "title" : "SAMsynthetase and R0010, the neverending story", "content" : "First of all Gabriele quantified the four pSB1C3+R0010 inocula of yesterday.
Quantification
Sample | Quantity (ng/µl) |
---|---|
#A | 243.7 |
#B | 194.1 |
#C | 245.3 |
#D | 252.9 |
Then, the four quantified samples were screened after a digestion with ExoRI-HF and PstI-HF, with the usual screening protocol (incubated only for 45min, since both the enzymes are HF).
The digestion products were then run on a gel with a transparent loading dye (30% glycerol): each loaded sample contained 16µl of the sample and 4µl of transparent loading dye. Both a 1kb normal ladder and a 100bp transparent ladder were loaded, also the first well was loaded with the usual loading dye.
Gel
Loading scheme |
---|
Loading dye |
Empty |
100bp ladder |
#A |
#B |
#C |
#D |
Empty |
Empty |
1kb ladder |
Finally, Emil prepared the inocula of the product of ligation of R0010 and SAMsynthetase (in pSB1A2: 6 inocula of the 1:1 ligation product and 3 of the 1:3 ligation producta).", "tags" : "SAMsynthetase-Plac" }