Team:UNITN-Trento/Notebook/Labposts/07/17

From 2013.igem.org

(Difference between revisions)

Revision as of 08:25, 3 October 2013

{ "date" : "2013-07-09", "author" : "Bruno", "title" : "Ripenator v1.0", "content" : "I set up a system to ripening or block ripening a set of green (immature) bananas.
The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected.
In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.", "tags" : "Ripenator-Mesa-Ethylene" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-20",
+	"date" : "2013-07-09",
-	"author" : "fabio-bruno",
+	"author" : "Bruno",
-	"title" : " blue and red light sensors!!",
+	"title" : "Ripenator v1.0",
-	"content" : "<html> this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula. <br>Second set yields are: <br>006a : 289 <br>006b : 409.3<br>016° : 188.8<br>016b :266.6<br>030a : 388.6<br>030b : 270<br>082a : 433.0<br>082b : 568<br>Third set yiealds are: <br>006: 321 ng/ul<br>016: 440.9 ng/ul<br>R0082: 441,5 ng/ul<br>030: 199,9 ng/ul<br>These ones haven’t been screened so far. <br>Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.</html>",
+	"content" : "<html>I set up a system to ripening or block ripening a set of green (immature) bananas. <br>The idea is to grow into a beaker our strain of E. coli that produces ethylene, and connect it to a laboratory dryer with bananas. In this way, E. coli (be stirred and 37 ° C), producing Ethylene, should increase the maturation of the banana in the dryer connected. <br>In addition, we have included in another beaker Mesa pure with LB, which should slow down the ripening at high concentration and should promote the ripening at low concentrations.</html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
+	"tags" : "Ripenator-Mesa-Ethylene"
 }