Team:UNITN-Trento/Notebook/Labposts/07/51

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(Difference between revisions)
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{
{
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"date" : "2013-07-04",
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"date" : "2013-07-24",
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"author" : "thomas",
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"author" : "fabio-bruno",
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"title" : "Ethylene production kinetic assay",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html>In order to see the progress of the production of ethylene in time, 7 ml of culture were grown until O.D. 600 reached 0,5. The culture was then induced with 70 ul arabinose (5mM) and putted with a stirrer into a sealed tube with a pierceable septum. The tube was then connected to the Micro GC (Agilent 3000A) and immersed in a thermal bath at 37&deg;C with magnetic stirring. A measurment was taken every hour for 8 hours. The results were not so exciting. The colony didn't produce any level ethylene and we have no idea why. Maybe the colony from which the inocula were done wasn't the right one. I'll try the experiment again the next monday! <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Apparatus image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/9/96/Tn-2013_EFE_kinetic_apparatus.jpg\" style =\"width: 450px\"></center></html>}}<html></html>",
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"content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate  and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>",
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"tags" : "EFE"
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"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
}
}

Revision as of 08:38, 3 October 2013

{ "date" : "2013-07-24", "author" : "fabio-bruno", "title" : " the blue ligation: the never ending story !", "content" : "Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate and 2 from the 1:3 plate)
I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set).
After that I screened them all and found out that only the blue part was confirmed: up to now we only have the blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !!

Immagine gel
", "tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR" }

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