Team:UNITN-Trento/Notebook/Labposts/07/52

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{
{
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"date" : "2013-07-08",
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"date" : "2013-07-24",
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"author" : "thomas",
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"author" : "gabriele",
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"title" : "Ethylene production kinetic assay (second trial)",
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"title" : "Inocula",
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"content" : "<html>This morning I repeated <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-04-thomas\">the experiment</a> done the last thursday. This time however, I used the same vial used during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-06-28-thomas\">ethylene detection experiment</a>. This time the experiment worked as expected and I successfully plotted the data.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-2013_kinetic_plot-1.png\" style =\"width: 450px\"></center></html>}}<html>A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). </html>",
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"content" : "<html>I checked the plates prepared by Caterina <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-23-caterina\">yesterday</a>.<br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th><th>#inocula</th></tr><tr><td>ON Ctrl</td><td>0</td><td>0</td></tr><tr><td>ON 1:1</td><td>6</td><td>5</td></tr><tr><td>ON 1:3</td><td>0</td><td>0</td></tr><tr><td>Short Ctrl</td><td>0</td><td>0</td></tr><tr><td>Short 1:1</td><td>1</td><td>1</td></tr><tr><td>Short 1:3</td><td>1</td><td>1</td></tr></table></center><br/>As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.</html>",
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"tags" : "EFE"
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"tags" : "SAMsynthetase"
}
}

Revision as of 08:38, 3 October 2013

{ "date" : "2013-07-24", "author" : "gabriele", "title" : "Inocula", "content" : "I checked the plates prepared by Caterina yesterday.

plate#colonies#inocula
ON Ctrl00
ON 1:165
ON 1:300
Short Ctrl00
Short 1:111
Short 1:311

As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.", "tags" : "SAMsynthetase" }