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Team:UCL/Labbook/Week7
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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week19"> Week 19</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week20"> Week 20</a> | <p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week19"> Week 19</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week20"> Week 20</a> | ||
</p> | </p> | ||
| + | </div> | ||
| + | |||
| + | <div class="gap"> | ||
| + | </div> | ||
| + | |||
| + | <p class="major_title">July</p> | ||
| + | |||
| + | <div class="gap"> | ||
| + | </div> | ||
| + | |||
| + | <div class="full_row"> | ||
| + | <p class="minor_title">Week 7</p> | ||
| + | <p class="body_text"> | ||
| + | <b>Bacterial Lab</b> | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments. | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 16th July - <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C. | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours. | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C. | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 19th July - Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C. | ||
| + | </p> | ||
| + | </div> | ||
| + | |||
| + | <div class="full_row"> | ||
| + | <p class="body_text"> | ||
| + | <b>Mammalian Lab</b> | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 17th July - <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Mammalian cell culture and maintenance</a> training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3. | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 18th July - MEF passage 4 | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | 19th July - MEF passage 5 | ||
| + | </p> | ||
| + | </p> | ||
</div> | </div> | ||
Revision as of 10:37, 3 October 2013
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20
July
Week 7
Bacterial Lab
15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
16th July - 5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.
17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
Mammalian Lab
17th July - Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
18th July - MEF passage 4
19th July - MEF passage 5
