Team:UNITN-Trento/Notebook/Labposts/07/52

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{"date" : "2013-07-23","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "<html> this morning I added Sap to the 016 digestion and dPnl to the other two, then I purified them and quantified'em: <br>O16: 29,8 ng/ul <br>006: 16,2 ng/ul <br>R0010: 6,1 ng/ul <br>Then I started the ligation following the ligation protocol: at the end of it, I transformed 10ul of the ligation in 200 Neb10b and plated.Meanwhile Bruno miniprepped k952003 inocula and the screening was successful!!</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR- YF1_FixJ_PfixK2_amilGFP "}
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"date" : "2013-07-08",
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"author" : "thomas",
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"title" : "Ethylene production kinetic assay (second trial)",
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"content" : "<html>This morning I repeated <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-04-thomas\">the experiment</a> done the last thursday. This time however, I used the same vial used during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-06-28-thomas\">ethylene detection experiment</a>. This time the experiment worked as expected and I successfully plotted the data.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-2013_kinetic_plot-1.png\" style =\"width: 450px\"></center></html>}}<html>A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). </html>",
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"tags" : "EFE"
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}
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Latest revision as of 10:44, 3 October 2013

{"date" : "2013-07-23","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " this morning I added Sap to the 016 digestion and dPnl to the other two, then I purified them and quantified'em:
O16: 29,8 ng/ul
006: 16,2 ng/ul
R0010: 6,1 ng/ul
Then I started the ligation following the ligation protocol: at the end of it, I transformed 10ul of the ligation in 200 Neb10b and plated.Meanwhile Bruno miniprepped k952003 inocula and the screening was successful!!","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR- YF1_FixJ_PfixK2_amilGFP "}

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