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Team:UNITN-Trento/Notebook/Labposts/07/58
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| - | { | + | {"date" : "2013-07-24","author" : "thomas","title" : "Screening of AraC-pBAD + EFE + Venus","content" : "<html>This is the part five of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded with a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep purification</a> of the previously prepared inocula and a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">screening digestion</a> in order to verify the construct. <br/><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn-sp-table\"><tr><th>Sample</th><th>Concentration</th></tr><tr><td>A from ligation 1:1 </td><td>852.6 ng/ul</td></tr><tr><td>B from ligation 1:1 </td><td>1123 ng/ul</td></tr><tr><td>C from ligation 1:4 </td><td>685.8 ng/ul</td></tr><tr><td>D from ligation 1:4</td><td>2128.3 ng/ul</td></tr><tr><td>E from ligation 1:1 </td><td>1760 ng/ul</td></tr><tr><td>A from ligation 1:1 </td><td>852.6 ng/ul</td></tr></TABLE></center></html>}}<html> <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/d/d9/Tn-2013_venus_screening.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the gel image, only one sample seems to confirm our fusion protein and will be sent for sequencing. Lane 4: insert 3024 bp, vector 2070 bp. Kapa universal ladder was adopted.</html>","tags" : "EFE-Venus"} |
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Latest revision as of 10:46, 3 October 2013
{"date" : "2013-07-24","author" : "thomas","title" : "Screening of AraC-pBAD + EFE + Venus","content" : "This is the part five of this experiment. I proceeded with a miniprep purification of the previously prepared inocula and a screening digestion in order to verify the construct.
Quantification Results
| Sample | Concentration |
|---|---|
| A from ligation 1:1 | 852.6 ng/ul |
| B from ligation 1:1 | 1123 ng/ul |
| C from ligation 1:4 | 685.8 ng/ul |
| D from ligation 1:4 | 2128.3 ng/ul |
| E from ligation 1:1 | 1760 ng/ul |
| A from ligation 1:1 | 852.6 ng/ul |
As you can see from the gel image, only one sample seems to confirm our fusion protein and will be sent for sequencing. Lane 4: insert 3024 bp, vector 2070 bp. Kapa universal ladder was adopted.","tags" : "EFE-Venus"}
![](\"https://static.igem.org/mediawiki/2013/d/d9/Tn-2013_venus_screening.JPG\")
