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GigiGoesGugu (Talk | contribs) |
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'''Cell Surface Display''' | '''Cell Surface Display''' | ||
| - | ''PCR optimisation | + | ''PCR optimisation'' <BR> |
| + | To improve the ''pINP_construct'' amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. | ||
we verified our results with a 0.8% gel electrophoresis. | we verified our results with a 0.8% gel electrophoresis. | ||
| + | <BR><BR><BR> | ||
'''General''' | '''General''' | ||
PCR optimisation
To improve the pINP_construct amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix.
we verified our results with a 0.8% gel electrophoresis.
General
Competent cells try 3: it worked!
"
