GigiGoesGugu (Talk | contribs) |
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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
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''PCR optimisation'' <BR> | ''PCR optimisation'' <BR> | ||
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Competent cells try 3: it worked! | Competent cells try 3: it worked! | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
PCR optimisation
To improve the pINP_construct amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix.
we verified our results with a 0.8% gel electrophoresis.
General Competent cells try 3: it worked!
"
