GigiGoesGugu (Talk | contribs) |
GigiGoesGugu (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | + | <font size = "4">Cell Surface Display</font> | |
Our Primers arrived today. <BR> | Our Primers arrived today. <BR> | ||
| Line 7: | Line 7: | ||
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
<BR><BR><BR> | <BR><BR><BR> | ||
| - | + | <font size = "4">General</font> | |
| - | + | ||
Competent cells try 2. | Competent cells try 2. | ||
PCR
We did a PCR (see protocols) to amplifay pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
