Team:EPF Lausanne/Calendar/6 August 2013

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<font size = "4"> Cell Surface Display </font>
<font size = "4"> Cell Surface Display </font>
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''Gibson Transformation: ''  
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''Gibson Transformation '' <BR>
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<br>Transformation of HB5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
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<br>-Transformation of HB5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
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<br>Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
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<br>-Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
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<BR>-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.
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Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.
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<font size = "4"> Nanoparticles </font>
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'''Nanoparticles'''
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<br>- Purification of the digested GPs.
<br>- Purification of the digested GPs.

Revision as of 14:56, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Transformation

-Transformation of HB5α competent cells with INP_Strep-Dead gibson product previously obtained.
-Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.

Nanoparticles


- Purification of the digested GPs.
- Measurement of the GPs+dye with Dynamic Light Scattering (DLS). Results: good, we get some 10nm particles and a few 100nm. We hope they are nanoparticles and not artifacts.
- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/6_August_2013"