GigiGoesGugu (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the BBa_J23119 part from iGEM''' <BR> In order to have a positive control for our sensing constructs we wanted to use a const...") |
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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | <font size = "4"> Sensing </font> <BR> | ||
| - | + | ''PCR of the BBa_J23119 part from iGEM'' <BR> | |
| - | + | -In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid. <BR> | |
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| - | In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid. <BR> | + | |
The PCR of this promoter worked very well. | The PCR of this promoter worked very well. | ||
PCR of the BBa_J23119 part from iGEM
-In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid.
The PCR of this promoter worked very well.
"
