Team:EPF Lausanne/Calendar/15 September 2013

From 2013.igem.org

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Sensing <BR>
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<font size = "4"> Sensing </font> <BR>
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'''Analysis of the sequencing resutls of the three promoters (sensing-module)''' <BR>
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''Analysis of the sequencing resutls of the three promoters (sensing-module)'' <BR>
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The sequencing results were rather promising except for the Hya promoter. The sample that I send did not contain it. So I purified one in order to send it again on Tuesday. This sample was from one of the assays. I send this sample because it did not turn green when I added arabinose indicating that the Arabinose inducible promoter had effectively been removed and hopefully replaced by the hya promoter.
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-The sequencing results were rather promising except for the Hya promoter. The sample that I send did not contain it. So I purified one in order to send it again on Tuesday. This sample was from one of the assays. I send this sample because it did not turn green when I added arabinose indicating that the Arabinose inducible promoter had effectively been removed and hopefully replaced by the hya promoter.
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'''Functional assays for the three promoters (sensing-module)'''<BR>
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''Functional assays for the three promoters (sensing-module)''<BR>
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I again tried to grow the bacteria in an LB medium to which I added different amounts of either 10X MOPS or 10X HEPES buffer. But again the cultures did not turn green.
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-I again tried to grow the bacteria in an LB medium to which I added different amounts of either 10X MOPS or 10X HEPES buffer. But again the cultures did not turn green.
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'''MinPrep of the hya-plasmid''' <BR>
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''MinPrep of the hya-plasmid'' <BR>
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Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.
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-Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.
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<BR><BR>
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<font size = "4"> Nanoparticles</font> <BR>
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''Digestion assays''<BR>
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'''Nanoparticles'''
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''Culture of MMP2 and gelE from Sensing group''<BR>
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<BR>- Digestion assays
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<BR>- Culture of MMP2 and gelE from Sensing group
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Revision as of 16:54, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Analysis of the sequencing resutls of the three promoters (sensing-module)
-The sequencing results were rather promising except for the Hya promoter. The sample that I send did not contain it. So I purified one in order to send it again on Tuesday. This sample was from one of the assays. I send this sample because it did not turn green when I added arabinose indicating that the Arabinose inducible promoter had effectively been removed and hopefully replaced by the hya promoter.

Functional assays for the three promoters (sensing-module)
-I again tried to grow the bacteria in an LB medium to which I added different amounts of either 10X MOPS or 10X HEPES buffer. But again the cultures did not turn green.


MinPrep of the hya-plasmid
-Because the sequencing results showed that the plasmid I sent did not contain the hya promoter I picked another colony that I incubated and from which I then isolated the plasmid again by a MiniPrep.

Nanoparticles

Digestion assays

Culture of MMP2 and gelE from Sensing group

Wiki Links

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