Team:Braunschweig/Notebook

From 2013.igem.org

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     <h2><a href="#Week17">Week 17: September 8 - September 14, 2013</a></h2>
     <h2><a href="#Week17">Week 17: September 8 - September 14, 2013</a></h2>
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Week 17</p>
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<p style=" margin-left:5px; margin-right:5px;">We did some unseccessful growth curve experiments, but gain a lot of new infomation about our final construct. Te Experiments showed that bacteria carrying the final construct can grow on a low level on medium containing ampicillin without inducing the ampicillin resistance by HSL. But we figured out how to deal with this problem: We have to add the beta-lactamase inhibitor clavulanic acid to the culture broth to inhibit the antibiotic resistance just enough to allow for bacteria with induced resistance to grow, but prevents growth of non-induced cultures. Thus the next step for us is to determine the right beta-lactamase inhibitor concentration for our experiments. </p>
<p style=" margin-left:5px; margin-right:5px;">We did some unseccessful growth curve experiments, but gain a lot of new infomation about our final construct. Te Experiments showed that bacteria carrying the final construct can grow on a low level on medium containing ampicillin without inducing the ampicillin resistance by HSL. But we figured out how to deal with this problem: We have to add the beta-lactamase inhibitor clavulanic acid to the culture broth to inhibit the antibiotic resistance just enough to allow for bacteria with induced resistance to grow, but prevents growth of non-induced cultures. Thus the next step for us is to determine the right beta-lactamase inhibitor concentration for our experiments. </p>

Revision as of 17:43, 3 October 2013

Labjournal

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This is the documentation of our lab work. An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.

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