Team:Braunschweig/Notebook

From 2013.igem.org

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More digestions were set up to harvest the separated ampicillin resistance gene and prepare DNA for the next cloning round. Additionally, lactonase (C0060) was ligated with a RBS (B0032), the <i>ampR</i> gene was cloned behind our inducible promoters and we ligated each autoinducer synthetase with a RBS (B0032) overnight.
More digestions were set up to harvest the separated ampicillin resistance gene and prepare DNA for the next cloning round. Additionally, lactonase (C0060) was ligated with a RBS (B0032), the <i>ampR</i> gene was cloned behind our inducible promoters and we ligated each autoinducer synthetase with a RBS (B0032) overnight.
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<p><img alt="grey line" src="https://static.igem.org/mediawiki/2013/4/4c/Braunschweig_grey_line.png" width="850" height="1" vspace="20"/></p>
<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Friday, June 21, 2013</p>
<p style="font-size:14px; font-weight:bold; text-decoration:none; border:none; color:#be1e3c; margin-left:5px; margin-right:5px; margin-top:0px; margin-bottom:0px">Friday, June 21, 2013</p>

Revision as of 20:23, 3 October 2013

Labjournal

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This is the documentation of our lab work. An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.

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