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To improve the ''pINP_construct'' amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. | To improve the ''pINP_construct'' amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. | ||
we verified our results with a 0.8% gel electrophoresis. | we verified our results with a 0.8% gel electrophoresis. | ||
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<font size = "4">General</font> | <font size = "4">General</font> | ||
PCR optimisation
To improve the pINP_construct amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix.
we verified our results with a 0.8% gel electrophoresis.
General
Competent cells try 3: it worked!
"
