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<font size = "4">Cell Surface Display</font> | <font size = "4">Cell Surface Display</font> | ||
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The previous PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C. | The previous PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C. | ||
A 0.8% Electrophoresis gel was performed to verify the reation's products. | A 0.8% Electrophoresis gel was performed to verify the reation's products. | ||
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<font size = "4">Nanoparticles</font> | <font size = "4">Nanoparticles</font> | ||
Cell Surface Display
PCR optimisation
The previous PCR we did to amplify pINP_construct was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C.
A 0.8% Electrophoresis gel was performed to verify the reation's products.
Nanoparticles
Making Nanoparticles, 1st try
We added the cargo molecule (food dye) to GPs and left them for stirring (during 3 days)).
"
