Team:Braunschweig/Notebook

From 2013.igem.org

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<b>Investigators: Jan, Anna, Melanie</b><br>
<b>Investigators: Jan, Anna, Melanie</b><br>
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We conducted a cultivation experiment with the <i>E. Coli</i> JM109 bearing the final P<sub>Las</sub> construct to test the growth in presence and Absence of synthetic N-3-oxododecanoyl-HSL autoinducer added to the medium. Cultivation was carried out in four 500 mL non-baffled flasks with 75 mL 2xYT medium with ampicillin. In two flasks N-3-oxododecanoyl-HSL was added. Ideally  the flask without N-3-oxododecanoyl-HSL would not show any growth in the beginning until the antibiotic has been depleted. The N-3-oxododecanoyl-HSL in the other flask should induce the quorum-sensing controlled ampicillin resistance. Unfortunately all cultures were growing equally fast from the start so the experiment was aborted.</p>
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We conducted a cultivation experiment with the <i>E. Coli</i> JM109 bearing the final P<sub>Las</sub> construct to test the growth in presence and Absence of synthetic N-3-oxododecanoyl-HSL autoinducer added to the medium. Cultivation was carried out in four 500 mL non-baffled flasks with 75 mL 2xYT medium with ampicillin. In two flasks N-3-oxododecanoyl-HSL was added. Ideally  the flask without N-3-oxododecanoyl-HSL would not show any growth in the beginning until the antibiotic has been depleted. The N-3-oxododecanoyl-HSL in the other flask should induce the quorum sensing controlled ampicillin resistance. Unfortunately all cultures were growing equally fast from the start so the experiment was aborted.</p>
<p><img alt="grey line" src="https://static.igem.org/mediawiki/2013/4/4c/Braunschweig_grey_line.png" width="850" height="1" vspace="20"/></p>
<p><img alt="grey line" src="https://static.igem.org/mediawiki/2013/4/4c/Braunschweig_grey_line.png" width="850" height="1" vspace="20"/></p>

Revision as of 20:55, 3 October 2013

Labjournal

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This is the documentation of our lab work. An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our Attributions section for efforts beyond the lab work.

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