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Team:Heidelberg/Templates/MM week14
From 2013.igem.org
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Nils.kurzawa (Talk | contribs) m (Created page with " == 2013-07-29 == * inoculate 10 ml 2xYT+Amp with TOP10-pKD46, grow at 30°C == 2013-07-31 == [[File:PKD46-miniPrep-2013-07-31.png|100px|thumb|left|miniPrep of pKD46. Lane 1: NE...") |
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== 2013-07-29 == | == 2013-07-29 == | ||
* inoculate 10 ml 2xYT+Amp with TOP10-pKD46, grow at 30°C | * inoculate 10 ml 2xYT+Amp with TOP10-pKD46, grow at 30°C | ||
== 2013-07-31 == | == 2013-07-31 == | ||
| - | [[File: | + | [[File:Heidelberg_PKD46-miniPrep-2013-07-31.png|100px|thumb|left|miniPrep of pKD46. Lane 1: NEB 2-log (5 µl), lane 2: 1 µl of miniPrep]] |
* miniPrep of pKD46 -> ca. 10 ng/µl | * miniPrep of pKD46 -> ca. 10 ng/µl | ||
== 2013-08-03 == | == 2013-08-03 == | ||
* transform BAP1 with 5µl of pKD46 miniPrep form 2013-07-31, plate on Amp, grow at 30°C | * transform BAP1 with 5µl of pKD46 miniPrep form 2013-07-31, plate on Amp, grow at 30°C | ||
Revision as of 00:36, 4 October 2013
2013-07-29
- inoculate 10 ml 2xYT+Amp with TOP10-pKD46, grow at 30°C
2013-07-31
- miniPrep of pKD46 -> ca. 10 ng/µl
2013-08-03
- transform BAP1 with 5µl of pKD46 miniPrep form 2013-07-31, plate on Amp, grow at 30°C
