Team:TU Darmstadt/protocols/Heat Shock Transformation

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Revision as of 15:28, 4 October 2013





Heat Shock Transformation

Materials
 Ice
Heatingbad
Pipets + steril tips
DYT-Media
LB-Agar-Plates + antibiotics
Incubator

Procedure
 1. Defrost stocks of competent cells (100 µL in 1.5 ml Eppendorf tube) on ice
2. Add the DNA and incubate the suspension for 5 minutes on ice
3. Heat shock is done by incubating the cells for 1 minute at 42°C
4. Add 1 mL of DYT medium and incubate for 1 hour at 37°C in order to obtain antibiotic resistance
5. Finally the cells are ready to be crossed out

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation"