Team:Wageningen UR/Summary

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Revision as of 17:23, 4 October 2013

Summary

Subtitle

Concluding our iGEM project in 2013, we introduced Aspergillu Niger as a potential host into iGEM. With the host engineering, we stepped further to the next level of synthetic biology. In this GRAS organism, we established a modular system of domain shuffling in order to express a variety of secondary metabolites. The lovastatin as a crucial compound in medical industry was further studied and the domains of its key enzyme was assembled and modular. Via the modularity approach, their physiology was modified to some extent to comply the need of industry. Additionally, to target the production of specific compartment, a toolkit including pH sensor, ATP sensor and visible chromoprotein display was created. It accelerated the exploration of more potentiality.

For the further study, we recommend to make the toolkit more completed and increase the repertoire with new part and devices. And our mix-match assembling method can be better improved to produce more valuable secondary metabolites.

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