Team:Baskent Meds/Project

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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!align="center"|[[Team:Baskent_Meds|Home]]
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!align="center"|[[Team:Baskent_Meds/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Baskent_Meds Official Team Profile]
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!align="center"|[[Team:Baskent_Meds/Project|Project]]
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!align="center"|[[Team:Baskent_Meds/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Baskent_Meds/Modeling|Modeling]]
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!align="center"|[[Team:Baskent_Meds/Notebook|Notebook]]
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!align="center"|[[Team:Baskent_Meds/Safety|Safety]]
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!align="center"|[[Team:Baskent_Meds/Attributions|Attributions]]
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== '''Overall project''' ==
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
 
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<body>
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== Project Details==
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<div id="ana" style="position:absolute; left:-24px; top:-100px; width:1000px; height:1100px; z-index:1; background-color: #FFFFFF; layer-background-color: #FFFFFF; border: 1px none #000000;">
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<article>
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<ul style="font-weight: bold;" class="topnav">
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<li><a href="/Team:Baskent_Meds" title="BaskentMeds main page">Home</a></li>
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=== Part 2 ===
 
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<li><a href="/Team:Baskent_Meds/Project" title="Our Project Abstract">Project</a>
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<ul style="font-weight: bold;" class="subnav">
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<li><a href="/Team:Baskent_Meds/Description" title="Project Description">Description</a></li>
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<li><a href="/Team:Baskent_Meds/Parts" title="Parts">Parts</a></li>
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<li><a href="/Team:Baskent_Meds/Lpdetect" title="Project Description">Legionella detection</a></li>
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<li><a href="/Team:Baskent_Meds/Notebook" title="Our Beloved Notebook!">Notebook</a></li>
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</ul>
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</li>
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<li><a href="/Team:Baskent_Meds/Team" title="Shall we meet?">Team</a>
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<ul class="subnav">
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<li><a href="/Team:Baskent_Meds/Team#fi" title="Our Instructors">Instructors</a></li>
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<li><a href="/Team:Baskent_Meds/Team#adv" title="Our Adisor">Advisor</a></li>
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<li><a href="/Team:Baskent_Meds/Team#us" title="Us">Student Members</a></li>
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</ul>
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=== The Experiments ===
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<li><a style="font-weight: bold;" href="/Team:Baskent_Meds/Gallery" title="For Fun!">Human Practice</a></li>
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<li><a style="font-weight: bold;" href ="/Team:Baskent_Meds/Attributions" title="Extras!">Extras</a>
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=== Part 3 ===
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<ul style="font-weight: bold;" class="subnav">
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<li><a href="/Team:Baskent_Meds/Safety" title="İs it safe">Safety</a></li>
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<li><a href="/Team:Baskent_Meds/Attributions" title="Attributions">Attributions</a></li>
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== Results ==
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<div id="sosyalkardesim" class="style2" style=" width:100px ;position: relative; left: 820px; top: -50px"><br>
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<a style="background: transparent" href="https://www.facebook.com/igembaskentmeds2013"><img width="26px" src="https://static.igem.org/mediawiki/2013/e/ef/Fbsa.png"></img></a>
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<a style="background: transparent" href="https://twitter.com/igembaskentmeds"><img width="26px" src="https://static.igem.org/mediawiki/2013/e/ee/Twiasd.png"></img></a>
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<a style="background: url(lock_icon.gif) transparent;" href="http://www.youtube.com/user/igembaskentmeds2013"><img width="40px" src="https://static.igem.org/mediawiki/2013/c/cd/Yubusa.png"></img></a>
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</div>
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<br />
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<br />
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<div id="la'book" class="style2" style="position:absolute; left:25px; top:165px; width:1000px; height:274px; z-index:1; overflow: visible;">
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  <div id="gecher" style="position:absolute; left:2px; top:0px; width:970px; height:468px; z-index:1">
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    <p class="style8" align="left"><strong>Transformation of Escherichia coli In Order To Develop Legionella pneumophila Sensing Bacteria</strong></p><br>
 +
 +
<p>In order to reduce the infection risk, detection of environmental source and reduction of microbial load are required. Hyperchlorination of water source and permanence of height water temperature provides mid-grade success. Besides, total eradication of Legionella pneumophila from water sources is quite difficult. Since organism’s potential of infection in closed water storage areas is low, reducing the number of organisms in water source would be a sufficient precaution for control. Our recombinant bacteria are able to respond to biological load of Legionella pneumophila so, increase in number of Legionella cells leads to increased secretion of anti-legionella peptide, and that plays an important role in controlling Legionella numbers in water source.
 +
</br>
 +
Legionella pneumophila cannot be cultured using conventional media, it needs mediums with cysteine and iron. Application of standard culture techniques and detection of bacteria from samples can last up to 10 days. Even identification at genus level is done on media, identification at species level is troubled and samples are generally sent to reference laboratories for this step. When detection with simultaneous quantitative polymerase chain reaction is considered, both method itself reduces required time and identification time is shortened by usage of commercial kits. However, in order this method to give accurate quantitative results at adequate titration, sensitive standardizations must be done for different types of environmental samples. This test is needed to be done in standardized reference laboratories and cost per sample is high. With our quorum sensing based system, presence of low bacterial load can be detected at species level.
 +
</br>
 +
In conclusion, detection and prevention of colonization of Legionella pneumophila are substantially costly and requires long processes. Our biological system can both provide detection and prevent colonization that occurred via high bacterial load with a considerably low cost. Since is works with natural biological expression process, the system can react to environmental alterations in a small amount of time. As the system can settle itself on colonization surfaces, it is easy to use after the first inoculation. The system does not constitute any risks in means of biosafety because Escherichia coli strains which are noninfectious, present in environment and has low virulence are manipulated and developed</p>
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<div id="Our Proudly Sponsors" class="style2" align="center">
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      </br>
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</br>
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</br>
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<p><strong>Our Sponsors</strong></p>
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<a href="http://www.baskent.edu.tr" title="Başkent Üniversitesi"><img src="https://static.igem.org/mediawiki/2013/3/30/Baskentlogo.gif" width="100" height="90" ></a><a href = "http://www.tubitak.gov.tr" title="TÜBİTAK"><img src="https://static.igem.org/mediawiki/2013/b/bf/Tubitaklogo.jpg" width="100" height="90"></a><a href = "http://www.home.agilent.com/agilent/home.jspx?lc=eng&cc=TR" title="Agilent Türkiye"><img src="https://static.igem.org/mediawiki/2013/c/c3/Aglientsmszw.png" width="100" height="90"></a>
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</br>
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</br>
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</br></div>
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</div>

Latest revision as of 18:23, 4 October 2013

Baskent_Meds IGEMwiki



Transformation of Escherichia coli In Order To Develop Legionella pneumophila Sensing Bacteria


In order to reduce the infection risk, detection of environmental source and reduction of microbial load are required. Hyperchlorination of water source and permanence of height water temperature provides mid-grade success. Besides, total eradication of Legionella pneumophila from water sources is quite difficult. Since organism’s potential of infection in closed water storage areas is low, reducing the number of organisms in water source would be a sufficient precaution for control. Our recombinant bacteria are able to respond to biological load of Legionella pneumophila so, increase in number of Legionella cells leads to increased secretion of anti-legionella peptide, and that plays an important role in controlling Legionella numbers in water source.
Legionella pneumophila cannot be cultured using conventional media, it needs mediums with cysteine and iron. Application of standard culture techniques and detection of bacteria from samples can last up to 10 days. Even identification at genus level is done on media, identification at species level is troubled and samples are generally sent to reference laboratories for this step. When detection with simultaneous quantitative polymerase chain reaction is considered, both method itself reduces required time and identification time is shortened by usage of commercial kits. However, in order this method to give accurate quantitative results at adequate titration, sensitive standardizations must be done for different types of environmental samples. This test is needed to be done in standardized reference laboratories and cost per sample is high. With our quorum sensing based system, presence of low bacterial load can be detected at species level.
In conclusion, detection and prevention of colonization of Legionella pneumophila are substantially costly and requires long processes. Our biological system can both provide detection and prevent colonization that occurred via high bacterial load with a considerably low cost. Since is works with natural biological expression process, the system can react to environmental alterations in a small amount of time. As the system can settle itself on colonization surfaces, it is easy to use after the first inoculation. The system does not constitute any risks in means of biosafety because Escherichia coli strains which are noninfectious, present in environment and has low virulence are manipulated and developed




Our Sponsors




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