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Team:Braunschweig
From 2013.igem.org
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<h1>Our project</h1> | <h1>Our project</h1> | ||
<p><img alt="linie rot 8pix hoch" src="https://static.igem.org/mediawiki/2013/0/07/Team_Braunschweig_Red_line.jpg" width="400" height="1" /></p> | <p><img alt="linie rot 8pix hoch" src="https://static.igem.org/mediawiki/2013/0/07/Team_Braunschweig_Red_line.jpg" width="400" height="1" /></p> | ||
| - | <img alt="modellvereinfacht" src="https://static.igem.org/mediawiki/2013/3/37/Braunschweig_mascotts.png" width="150" align="left" vspace="10" hspace="20" alt="Text?" /> <p style="text-align:justify; margin-left:5px; margin-right:5px;"> | + | <img alt="modellvereinfacht" src="https://static.igem.org/mediawiki/2013/3/37/Braunschweig_mascotts.png" width="150" align="left" vspace="10" hspace="20" alt="Text?" /> <p style="text-align:justify; margin-left:5px; margin-right:5px;">Bacterial consortia offer a great benefit for synthetic biology due to the ability to perform complex tasks by splitting the whole reaction into smaller reactions and share the task among different specialized strains. Also, a self-regulating bacterial culture with intra consortial dependencies offers great advances in biosafety. To shut down the whole bacterial consortium, only on strain has to be eliminated. We engineer three different E. coli strains to grow in a consortium exploiting different Quorum Sensing systems. Each strain maintains a constitutive expression of an inactive transcription activator (LuxR, LasR or RhlR). Inducers are synthesized by different synthases (LuxI, LasI or RhlI) that are each expressed in one strain and subsequently secreted into the medium. Once taken up by a cell, the inducers bind to the corresponding, inactive transcription factors to render them functional. As a result, an antibiotic resistance under the control of an inducible promoter is expressed. </p> |
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Revision as of 19:00, 4 October 2013
Welcome
Our project
Bacterial consortia offer a great benefit for synthetic biology due to the ability to perform complex tasks by splitting the whole reaction into smaller reactions and share the task among different specialized strains. Also, a self-regulating bacterial culture with intra consortial dependencies offers great advances in biosafety. To shut down the whole bacterial consortium, only on strain has to be eliminated. We engineer three different E. coli strains to grow in a consortium exploiting different Quorum Sensing systems. Each strain maintains a constitutive expression of an inactive transcription activator (LuxR, LasR or RhlR). Inducers are synthesized by different synthases (LuxI, LasI or RhlI) that are each expressed in one strain and subsequently secreted into the medium. Once taken up by a cell, the inducers bind to the corresponding, inactive transcription factors to render them functional. As a result, an antibiotic resistance under the control of an inducible promoter is expressed.
Our project
In nature microorganisms commonly live in symbiosis, be it in form of a protective biofilm or as composite organism[s] such as lichen (a symbiosis of algae/cyanobacteria and fungi). Members of this symbiotic community secrete chemicals that offer protection and nutrition or neutralize toxins.
You can find a more detailed project description here!
Our project
In nature microorganisms commonly live in symbiosis, be it in form of a protective biofilm or as composite organism[s] such as lichen (a symbiosis of algae/cyanobacteria and fungi). Members of this symbiotic community secrete chemicals that offer protection and nutrition or neutralize toxins.
You can find a more detailed project description here!
Our project
In nature microorganisms commonly live in symbiosis, be it in form of a protective biofilm or as composite organism[s] such as lichen (a symbiosis of algae/cyanobacteria and fungi). Members of this symbiotic community secrete chemicals that offer protection and nutrition or neutralize toxins.
You can find a more detailed project description here!
Our project
In nature microorganisms commonly live in symbiosis, be it in form of a protective biofilm or as composite organism[s] such as lichen (a symbiosis of algae/cyanobacteria and fungi). Members of this symbiotic community secrete chemicals that offer protection and nutrition or neutralize toxins.
You can find a more detailed project description here!
Our sponsors
