Team:UCL/Labbook/Week10

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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week19"> Week 19</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week20"> Week 20</a>
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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a>  
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<p class="minor_title">Week 10</p>
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<div class="full_row">
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<div class="gap">
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</div>
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<p class="body_text">
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<b>Bacterial Lab</b>
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</p>
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<p class="body_text">
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<b>Monday 5th August</b>
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</p>
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<p class="body_text">
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The new batch of <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> ampicillin</a> was tested via <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> transformation</a> of competent cells with pSecTag2A. Plates were <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> spread</a> and incubated at 37°C overnight.
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</p>
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<p class="body_text">
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<b>Tuesday 6th August</b>
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</p>
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<p class="body_text">
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Results from 5th August transformation:
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</p>
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<p class="body_text">
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<table>
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<tr>
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<th>Vial</th>
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<th>Ampicillin Plate</th>
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<th>Plasmid Insertion</th>
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<th>Colony Count</th>
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</tr>
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<tr>
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<td>1 (Main)</td>
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<td>Yes</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<tr>
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<td>2 (Positive Control)</td>
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<td>No</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<td>3 (Negative Control)</td>
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<td>Yes</td>
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<td>No</td>
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<td>15</td>
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</tr>
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</table>
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</p>
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<p class="body_text">
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This indicates that there is still an issue with the <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> ampicillin</a>, possibly a problem with the stock powder used. A final test with both old and new <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> ampicillin</a> was carried out and compared without plasmid insertion.
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</p>
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<p class="body_text">
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<b>Wednesday 7th August</b>
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</p>
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<p class="body_text">
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<table>
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<tr>
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<th>Vial</th>
 +
<th>Ampicillin Plate</th>
 +
<th>Plasmid Insertion</th>
 +
<th>Colony Count</th>
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</tr>
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<tr>
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<td>1 Old Ampicillin</td>
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<td>Yes</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<tr>
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<td>2 New Ampicillin v2</td>
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<td>No</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<td>3 Positive Control</td>
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<td>Yes</td>
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<td>No</td>
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<td>15</td>
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</tr>
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</table>
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</p>
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<p class="body_text">
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Results indicated that the <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> ampicillin</a> source may not have been fully functional, therefore a new source of ampicillin powder was located and ampicillin was remade.
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</p>
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<p class="body_text">
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<b>Thursday 8th August</b>
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</p>
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<p class="body_text">
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Preparation of 4x <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> ampicillin plates</a> and 4x <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> no drug plates</a> for storage in the fridge.
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</p>
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<p class="body_text">
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<b>Friday 9th August</b>
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</p>
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<p class="body_text">
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Meeting with supervisor Dr Darren Nesbeth
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</p>
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<p class="body_text">
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Goals to complete for upcoming weeks:
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</p>
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<p class="body_text">
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create <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> glycerol stocks</a> of both pSecTag2A and pSB1C3, purify and extract pure DNA for pSecTag2A and pSB1C3.
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</p>
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</div>
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<div class="gap">
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</div>
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<div class="full_row">
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<div class="gap">
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</div>
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<p class="body_text">
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<b>Mammalian Lab</b>
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</p>
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<p class="body_text">
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<b>Tuesday 6th August</b>
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</p>
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<p class="body_text">
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Checked HeLa cells. Cells are growing slow, left to grow for a few more days
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</p>
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<p class="body_text">
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<b>Thursday 8th August</b>
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</p>
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<p class="body_text">
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HeLa cells are about 30% confluent. Changed media.
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</p>
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</div>
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<!-- END CONTENT ------------------------------------------------------------------------------------------------------>
<!-- END CONTENT ------------------------------------------------------------------------------------------------------>

Latest revision as of 19:13, 4 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18

Week 10

Bacterial Lab

Monday 5th August

The new batch of ampicillin was tested via transformation of competent cells with pSecTag2A. Plates were spread and incubated at 37°C overnight.

Tuesday 6th August

Results from 5th August transformation:

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 100+
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 15

This indicates that there is still an issue with the ampicillin, possibly a problem with the stock powder used. A final test with both old and new ampicillin was carried out and compared without plasmid insertion.

Wednesday 7th August

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 Old Ampicillin Yes Yes 100+
2 New Ampicillin v2 No Yes 100+
3 Positive Control Yes No 15

Results indicated that the ampicillin source may not have been fully functional, therefore a new source of ampicillin powder was located and ampicillin was remade.

Thursday 8th August

Preparation of 4x ampicillin plates and 4x no drug plates for storage in the fridge.

Friday 9th August

Meeting with supervisor Dr Darren Nesbeth

Goals to complete for upcoming weeks:

create glycerol stocks of both pSecTag2A and pSB1C3, purify and extract pure DNA for pSecTag2A and pSB1C3.

Mammalian Lab

Tuesday 6th August

Checked HeLa cells. Cells are growing slow, left to grow for a few more days

Thursday 8th August

HeLa cells are about 30% confluent. Changed media.

Retrieved from "http://2013.igem.org/Team:UCL/Labbook/Week10"