"
Team:TU Darmstadt/labbook
From 2013.igem.org
(Difference between revisions)
| Line 252: | Line 252: | ||
<center> | <center> | ||
<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/labbook"> | ||
| - | <font size="8" color="#F0F8FF" face="Arial regular"> | + | <font size="8" color="#F0F8FF" face="Arial regular">Lab book |</font> |
</a> | </a> | ||
<a href="https://2013.igem.org/Team:TU_Darmstadt/materials"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/materials"> | ||
| Line 281: | Line 281: | ||
<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/DetectionConstruct"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/DetectionConstruct"> | ||
<img alt="Labbookdetection" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif"> | <img alt="Labbookdetection" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif"> | ||
| - | <font size="6" color="#F0F8FF" face="Arial regular">Visit Our | + | <font size="6" color="#F0F8FF" face="Arial regular">Visit Our lab book for the detection construct</font><br /> |
</a> | </a> | ||
| Line 311: | Line 311: | ||
<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/Biobricks"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/Biobricks"> | ||
<img alt="Labbookbiobricks" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif"> | <img alt="Labbookbiobricks" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif"> | ||
| - | <font size="6" color="#F0F8FF" face="Arial regular">Visit Our | + | <font size="6" color="#F0F8FF" face="Arial regular">Visit Our lab book for the fluorescence proteins</font><br /> |
</a> | </a> | ||
<br> | <br> | ||
Revision as of 19:40, 4 October 2013
.jpg){kind=small}
Detection construct
The assembly of our detection construct proved to be tricky. Not only the traditional cloning approach failed but also the assembly using synthesized gBlocks delivered no results. We finally turned to an In-Fusion cloning approach to assemble our construct. Visit our lab book to read more about our work flow.
Visit Our lab book for the detection constructFluorescence proteins
The genes for our FRET pair, mKate and LssmOrange, were isolated by PCR, cloned into the biobrick vector and sequenced afterwards. The genes were also cloned into a commercially available expression vector in order to characterize the fluorescent proteins. Visit our lab book to read more about our work flow.
Visit Our lab book for the fluorescence proteinsSafety
The construction of the pDawn vector from gBlocks failed.
Visit our lab book for the safety construct
