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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
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<font size = "4">Cell Surface Display</font> | <font size = "4">Cell Surface Display</font> | ||
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The previous PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C. | The previous PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C. | ||
A 0.8% Electrophoresis gel was performed to verify the reation's products. | A 0.8% Electrophoresis gel was performed to verify the reation's products. | ||
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<font size = "4">Nanoparticles</font> | <font size = "4">Nanoparticles</font> | ||
''Making Nanoparticles, 1st try'' <BR> | ''Making Nanoparticles, 1st try'' <BR> | ||
| - | We added the cargo molecule (food dye) to GPs and left them for stirring (during 3 days | + | We added the cargo molecule (food dye) to GPs and left them for stirring (during 3 days). |
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Cell Surface Display
PCR optimisation
The previous PCR we did to amplify pINP_construct was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74°C, 76°C and 78°C.
A 0.8% Electrophoresis gel was performed to verify the reation's products.
Nanoparticles
Making Nanoparticles, 1st try
We added the cargo molecule (food dye) to GPs and left them for stirring (during 3 days).
"
