Team:EPF Lausanne/Calendar/15 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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''BBa_K523013 Characterisation'' <br>
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Characterisation of the iGEM plasmid BBa_K523013 we decided to use.
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<br><br>
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'''Primer Resuspension''' <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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We recieved our primers and resuspended them. Then we dilluted them in order to have a final concentration of 100umol.
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'''Innoculating Bacteria''' <BR>
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''Primer Resuspension'' <BR>
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From each agar plate that we inncubated over-night we innoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.  
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-We received our primers and resuspended them. Then we diluted them in order to have a final concentration of 100umol.
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<BR>
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'''Nanoparticles'''
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''Inoculating Bacteria'' <BR>
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-From each agar plate that we incubated over-night we inoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.
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<BR> <BR>
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<font size = "4"> Nanoparicles</font> <BR>
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''Making Nanoparticles, 2nd try''
<BR>- The nanoparticles are done.
<BR>- The nanoparticles are done.
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<BR>- Characterization of the new gelatin nanoparticles with the DLS : their average size is 160 nm !  
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<BR>- Characterisation of the new gelatin nanoparticles with the DLS : their average size is 160 nm !  
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Latest revision as of 21:51, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector

Primer Resuspension
-We received our primers and resuspended them. Then we diluted them in order to have a final concentration of 100umol.

Inoculating Bacteria
-From each agar plate that we incubated over-night we inoculated one colony in order to make a glycerol stock of MMP2 and MMP9 containing bacteria the next day.

Nanoparicles

Making Nanoparticles, 2nd try
- The nanoparticles are done.
- Characterisation of the new gelatin nanoparticles with the DLS : their average size is 160 nm !


Wiki Links

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