Team:EPF Lausanne/Calendar/16 August 2013

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{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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''BBa_K523013 Characterisation'' <br>
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Characterisation of the iGEM plasmid BBa_K523013 we decided to use.
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<br><br>
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'''Making a glycerol stock''' <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 500ul 50% glycerol.
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''Making a glycerol stock'' <BR>
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-From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 500ul 50% glycerol.
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'''PCR'''<BR>
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''PCR''<BR>
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We Started the Gibson assembly of four of our constructs by making a PCR of each, the Insert as well as the Backbone, making eight different PCR tubes in total (see protocol PCR). About twenty minutes before the PCR reaction was done, the machine malfunctionned. We did run a 0.8% gel, but The results cannot be well interpreted, as the reaction could not go to completion.
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We Started the Gibson assembly of four of our constructs by making a PCR of each, the Insert as well as the Backbone, making eight different PCR tubes in total (see protocol PCR). About twenty minutes before the PCR reaction was done, the machine malfunctioned. We did run a 0.8% gel, but The results cannot be well interpreted, as the reaction could not go to completion.
We re-ran another PCR reaction with the same constructs, as well as a reactant control. After the PCR the amplified DNA was stored over night at 4°C.
We re-ran another PCR reaction with the same constructs, as well as a reactant control. After the PCR the amplified DNA was stored over night at 4°C.
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<BR> <BR>
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''Tubes:''
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<font size = "4"> Nanoparticles</font> <BR>
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1.) MMP2 insert with linker(5.4-I) <BR>
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2.) MMP2 insert without linker (4.4-I) <BR>
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''Making Nanoparticles, 2nd try'' <BR>
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3.) Backbone with GFP (5.4-B) <BR>
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- We washed the nanoparticles with 75% acetone.  
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4.) Backbone without GFP (4.4-B) <BR>
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1.) MMP9 insert with linker(7.4-I) <BR>
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2.) MMP9 insert without linker (6.4-I) <BR>
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3.) Backbone with GFP (7.4-B)<BR>
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4.) Backbone without GFP (6.4-B) <BR>
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Latest revision as of 21:51, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector
Making a glycerol stock
-From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 500ul 50% glycerol.

PCR
We Started the Gibson assembly of four of our constructs by making a PCR of each, the Insert as well as the Backbone, making eight different PCR tubes in total (see protocol PCR). About twenty minutes before the PCR reaction was done, the machine malfunctioned. We did run a 0.8% gel, but The results cannot be well interpreted, as the reaction could not go to completion. We re-ran another PCR reaction with the same constructs, as well as a reactant control. After the PCR the amplified DNA was stored over night at 4°C.

Nanoparticles

Making Nanoparticles, 2nd try
- We washed the nanoparticles with 75% acetone.



Wiki Links

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