Team:EPF Lausanne/Calendar/1 September 2013

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Sensing <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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'''Suspending Primers''' <BR>
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''Suspending Primers'' <BR>
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Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.
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-Our new primers arrived and we resuspended them and made primer mixes for each pair, one forward and one reverse.
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<BR>
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'''Suspending Primers''' <BR>
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''Inducing and measuring GFP expression'' <BR>
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The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.
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-The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.
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<BR>
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''PCR of Backbones'' <BR>
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-We tried to amplify the backbones of the sensing and effector constructs by using the new primers. It did not work.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 22:08, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing-Effector

Suspending Primers
-Our new primers arrived and we resuspended them and made primer mixes for each pair, one forward and one reverse.

Inducing and measuring GFP expression
-The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.

PCR of Backbones
-We tried to amplify the backbones of the sensing and effector constructs by using the new primers. It did not work.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/1_September_2013"