Team:EPF Lausanne/Calendar/13 September 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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''Inverted Microscopy'' <BR>
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- All our constructs (ISA,ISD,ISI and competent cells as negative control) were incubated 1hr with TexRed conjugate nanoparticles.
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<br>- Visualization under the microscope with a cy5 filter.
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<br><br>
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'''Innoculation of Bacteria with our constructs''' <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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In order to characterize both the sensing and the effector plasmid I innoculated Bacteria in liquid LB cultures over-night.
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''Innoculation of Bacteria with our constructs'' <BR>
 +
-In order to characterize both the sensing and the effector plasmid I inoculated Bacteria in liquid LB cultures over-night.
 +
<BR><BR>
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'''Nanoparticles'''
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<font size = "4"> Nanoparticles</font> <BR>
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<BR>- Biotinylation of the two new nanoparticles batches
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<BR>- End of the anti-strepta resistance check (sample mix, use of strepatavidin beads and microscopy)
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''Biotinylation''<BR>
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<BR>- DLS measurements of the new biotinylated nanoparticles (before dialysis)  
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-We biotinylated the two new nanoparticles batches.
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<BR>
 +
-End of the anti-strepta resistance check (sample mix, use of strepatavidin beads and microscopy)
 +
 
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''DSL Measurements''<BR>
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-We did DLS measurements of the new biotinylated nanoparticles (before dialysis).
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Latest revision as of 22:22, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Inverted Microscopy
- All our constructs (ISA,ISD,ISI and competent cells as negative control) were incubated 1hr with TexRed conjugate nanoparticles.
- Visualization under the microscope with a cy5 filter.

Sensing-Effector

Innoculation of Bacteria with our constructs
-In order to characterize both the sensing and the effector plasmid I inoculated Bacteria in liquid LB cultures over-night.

Nanoparticles

Biotinylation
-We biotinylated the two new nanoparticles batches.
-End of the anti-strepta resistance check (sample mix, use of strepatavidin beads and microscopy)

DSL Measurements
-We did DLS measurements of the new biotinylated nanoparticles (before dialysis).

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/13_September_2013"