Team:EPF Lausanne/Calendar/6 August 2013

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<font size = "4"> Cell Surface Display </font>
<font size = "4"> Cell Surface Display </font>
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''Gibson Transformation: ''  
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''Gibson Transformation ''  
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<br>Transformation of HB5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
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<br>-Transformation of DH5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
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<br>Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
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<br>-Overnight incubation of the transformed cells in chloramphenicol plates at 37°C.
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<BR>-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.
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<BR> <BR>
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Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.
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<font size = "4"> Nanoparticles </font>
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''Making Nanoparticles, 1st try''  
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'''Nanoparticles'''
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<br>- Purification of the digested GPs.
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<br>- Measurement of the GPs+dye with Dynamic Light Scattering (DLS). <BR> Results: good, we got some 10 nm particles and a few 100 nm. We hope they are nanoparticles and not artifacts.  
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- Purification of the digested GPs.
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<br>- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.
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- Measurement of the GPs+dye with Dynamic Light Scattering (DLS). Results: good, we get some 10nm particles and a few 100nm. We hope they are nanoparticles and not artifacts.  
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- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.
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Latest revision as of 22:30, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Transformation
-Transformation of DH5α competent cells with INP_Strep-Dead gibson product previously obtained.
-Overnight incubation of the transformed cells in chloramphenicol plates at 37°C.
-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.

Nanoparticles

Making Nanoparticles, 1st try
- Purification of the digested GPs.
- Measurement of the GPs+dye with Dynamic Light Scattering (DLS).
Results: good, we got some 10 nm particles and a few 100 nm. We hope they are nanoparticles and not artifacts.
- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/6_August_2013"