Team:EPF Lausanne/Calendar/17 September 2013

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{{Template:EPFL2013Header}}
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<font size = "4"> Cell surface display</font> <BR>
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New strategy:
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-Dpni digestion of the PCR product, that didn't look perfect on the gel, but might still be worth a try, especially since the time is running out, because the semester starts today.
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- Gibson assembly second try of the INP-YFP-Streptavidin construct and the INP-Streptavidin-YFP construct.
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<font size = "4"> Sensing-Effector </font> <BR>
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Sensing <BR>
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''Inoculation and glycerol stock'' <BR>
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-In the morning I inoculated the bacteria that were grown over night (with the hya-promoter, the cad-prmoter and the constitutive promoter). In the evening I then used this liquid cultures to do a glycerol stock of each construct as well as a MiniPrep of the remaining culture in order to have a plasmid stock.
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'''Innoculation and glycerol stock''' <BR>
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In the morning I innoculated the bacteria that were grown over night (with the hya-promoter, the cad-prmoter and the constitutive promoter). In the evening I then used this liquid cultures to do a glycerol stock of each construct as well as a MiniPrep of the remaining culture in order to have a plasmid stock.
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{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 22:49, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell surface display
New strategy: -Dpni digestion of the PCR product, that didn't look perfect on the gel, but might still be worth a try, especially since the time is running out, because the semester starts today. - Gibson assembly second try of the INP-YFP-Streptavidin construct and the INP-Streptavidin-YFP construct. Sensing-Effector

Inoculation and glycerol stock
-In the morning I inoculated the bacteria that were grown over night (with the hya-promoter, the cad-prmoter and the constitutive promoter). In the evening I then used this liquid cultures to do a glycerol stock of each construct as well as a MiniPrep of the remaining culture in order to have a plasmid stock.


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