Team:BYU Provo/Small Phage

From 2013.igem.org

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Overview
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Winter
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[[Team:BYU Provo/Notebook/SmallPhage/Springexp|Spring]]
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: [[Team:BYU_Provo/Small_Phage|Overview]]
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Summer
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: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]
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Fall
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: [[Team:BYU Provo/Notebook/SmallPhage/Springexp|May-June]]
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: [[Team:BYU Provo/Notebook/SmallPhage/Summerexp|July-August]]
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'''add overview'''
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Description of our purpose and approaches
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: [[Team:BYU Provo/Notebook/SmallPhage/Fallexp|September-October]]
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[[Spring]] [[Winter]]
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'''Things to figure out'''
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<font color="#333399" size="5" font face="Calibri"> '''Overview'''</font>
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The mission of Brigham Young University--founded, supported, and guided by The Church of Jesus Christ of Latter-day Saints--is to assist individuals in their quest for perfection and eternal life. That assistance should provide a period of intensive learning in a stimulating setting where a commitment to excellence is expected and the full realization of human potential is pursued.
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All instruction, programs, and services at BYU, including a wide variety of extracurricular experiences, should make their own contribution toward the balanced development of the total person. Such a broadly prepared individual will not only be capable of meeting personal challenge and change but will also bring strength to others in the tasks of home and family life, social relationships, civic duty, and service to mankind.
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In meeting these objectives BYU's faculty, staff, students, and administrators should be anxious to make their service and scholarship available to The Church of Jesus Christ of Latter-day Saints in furthering its work worldwide. In an era of limited enrollments, BYU can continue to expand its influence both by encouraging programs that are central to the Church's purposes and by making its resources available to the Church when called upon to do so.
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==March==
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We believe the earnest pursuit of this institutional mission can have a strong effect on the course of higher education and will greatly enlarge Brigham Young University's influence in a world we wish to improve.
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==April==
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==May==
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===5/1/13===
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- Commencement of spring!!!
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- Discussed goals and outlined plans for spring term
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===5/2/13===
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- Designed primers for amplifying and sequencing phage capsid protein
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- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
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===5/3/13===
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- Performed agar test, focusing primarily on ×8
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: [[5.3 T7 phage selection method test]]
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- Processed phage amplification
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: [[5.3 T7 phage amplification/purification]]
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===5/4/13===
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- Performed dilution series using stock 5.3 (-1 through -11)
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- Started two 5mL overnights of BL21
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===5/5/13===
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- Spot test using stock 5.3 and its dilution series (from 5.4)
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: [[5.3 T7 phage amplification/purification]]
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- Started liquid culture for purification team (at around noon)
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: 1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)
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- Started 5.5 amplification from a plaque test
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: [[5.5 Amplification from a plaque test]]
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===5/6/13===
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- Continued [[5.3 T7 phage amplification/purification]]
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- Performed liquid culture phage concentration test
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: [[5.6 T7+ Liquid Culture Phage Concentration Test]]
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===5/7/13===
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- Started two 5mL of E coli BL21 overnight
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- Designed procedure for applying mutagen and selecting for T7
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===5/8/13===
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- Went over procedure for applying mutagen and PCR with Dr. Grose
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- Performed spot tests under [[5.6 T7+ Liquid Culture Phage Concentration Test]]
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- Started two 5mL BL21 overnights
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- Learned how to create LB plates
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===5/9/13===
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- Practiced with ×6 and ×8 top agar
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: [[5.9 T7 selection method test #2]]
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- Started [[5.9 T7+ Liquid Culture Phage Concentration Test #2]]
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- Discussed plans and schedule for next week.
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===5/20/13===
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LP, XL
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- We performed T7 Mutagen Concentration Test
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: [[5.20 Mutagen Concentration Experiment]]
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- We performed T7 Minor Capsid Protein PCR
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: [[5.20 T7 Minor Capsid Protein PCR]]
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===5/21/13===
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XL
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- started two 5mL E coli BL21 overnight at around 7:00pm
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===5/22/13===
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- Continued with [[5.20 Mutagen Concentration Experiment|T7 Mutagen Concentration Test]]
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- Continued with [[5.20 T7 Minor Capsid Protein PCR|T7 Minor Capsid Protein PCR ]] by running an agarose gel to confirm that we got the desired PCR product.
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==June==
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==July==
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==August==
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==September==
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{{TeamBYUProvoFooter}}
{{TeamBYUProvoFooter}}

Latest revision as of 22:55, 14 July 2013

BYUSmallPlageFrontPage.JPG


Overview
March-April
May-June
July-August
September-October

Overview


ADD small Phage project overview

The mission of Brigham Young University--founded, supported, and guided by The Church of Jesus Christ of Latter-day Saints--is to assist individuals in their quest for perfection and eternal life. That assistance should provide a period of intensive learning in a stimulating setting where a commitment to excellence is expected and the full realization of human potential is pursued.

All instruction, programs, and services at BYU, including a wide variety of extracurricular experiences, should make their own contribution toward the balanced development of the total person. Such a broadly prepared individual will not only be capable of meeting personal challenge and change but will also bring strength to others in the tasks of home and family life, social relationships, civic duty, and service to mankind.

.....

In meeting these objectives BYU's faculty, staff, students, and administrators should be anxious to make their service and scholarship available to The Church of Jesus Christ of Latter-day Saints in furthering its work worldwide. In an era of limited enrollments, BYU can continue to expand its influence both by encouraging programs that are central to the Church's purposes and by making its resources available to the Church when called upon to do so.

We believe the earnest pursuit of this institutional mission can have a strong effect on the course of higher education and will greatly enlarge Brigham Young University's influence in a world we wish to improve.



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