Team:UCL/Labbook/Week7

From 2013.igem.org

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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a>
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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a>  
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Monday 15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
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<b>Monday 15th July</b>
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Tuesday 16th July - <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>,  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C.  
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The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.  
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Wednesday 17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
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<b>Tuesday 16th July</b>
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Thursday 18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.  
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<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C.  
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Friday 19th July - Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.  
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<b>Wednesday 17th July</b>
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Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
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<b>Thursday 18th July</b>
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Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul  <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.  
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<b>Friday 19th July</b>
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Sunday 22nd July -<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> of our <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out.  
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Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.  
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Wednesday 17th July -  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Mammalian cell culture and maintenance</a> training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
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<b>Wednesday 17th July</b>
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Thursday 18th July - MEF passage 4
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<a href="https://2013.igem.org/Team:UCL/Project/Protocols"> Mammalian cell culture and maintenance</a> training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
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Friday 19th July - MEF passage 5
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<b>Thursday 18th July</b>
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MEF passage 4
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22nd July - MEF passage 6. Training ends.
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<b>Friday 19th July</b>
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MEF passage 5
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Latest revision as of 23:44, 4 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18

July

Week 7

Bacterial Lab

Monday 15th July

The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.

Tuesday 16th July

5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.

Wednesday 17th July

Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.

Thursday 18th July

Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.

Friday 19th July

Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.

Mammalian Lab

Wednesday 17th July

Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.

Thursday 18th July

MEF passage 4

Friday 19th July

MEF passage 5

Retrieved from "http://2013.igem.org/Team:UCL/Labbook/Week7"