Team:UCL/Labbook

From 2013.igem.org

(Difference between revisions)
 
(288 intermediate revisions not shown)
Line 6: Line 6:
<script type="text/javascript" src="https://2013.igem.org/Team:UCL/static/head.js?action=raw&ctype=text/javascript">  
<script type="text/javascript" src="https://2013.igem.org/Team:UCL/static/head.js?action=raw&ctype=text/javascript">  
</script>
</script>
 +
 +
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:UCL/static/protocolstyles.css?action=raw&ctype=text/css" />
<script>
<script>
Line 38: Line 40:
<div id="container">
<div id="container">
<!-- START CONTENT ---------------------------------------------------------------------------------------------------->
<!-- START CONTENT ---------------------------------------------------------------------------------------------------->
-
<div class="full_page">
+
<div class="gap">
 +
</div>
-
<div class="main_image"></div>
+
<p class="major_title">Lab Weeks</p>
-
<p class="major_title">June</p>
+
<div class="gap">
 +
</div>
-
<p class="minor_title">Week 1-3</p>
+
<div class="full_page">
-
<p class="body_text">
+
<p class="body_text"><a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a>
-
No lab work
+
</p>
-
</p>
+
</div>
-
<p class="minor_title">Week 4</p>
+
<div class="full_row">
 +
<div class="gap">
 +
</div>
<p class="body_text">
<p class="body_text">
-
26th June - We are given safety training in the Advanced Centre for Biochemical Engineering in all relevant laboratories, as well as general procedures in case of emergency.
+
Click the links above to view the corresponding Lab diary weeks
-
</p>
+
</p>  
-
<p class="major_title">July</p>
+
</div>
-
<p class="minor_title">Week 5-6</p>
+
-
<p class="body_text">
+
-
No lab work
+
-
</p>
+
-
 
+
-
<p class="minor_title">Week 7</p>
+
-
<p class="body_text">
+
-
Bacterial Lab
+
-
</p>
+
-
15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
+
-
</p>
+
-
16th July - 5X M9 salts [link to protocol], minimal agar [link to protocol], 1.4% molten agar solution [link to protocol] and 0.1M CaCl2/15% glycerol [link to protocol] were prepared for the generation of competent cells. Minimal agar plates were poured and streaked [link to streaking protocol] with W3110 Escherichia coli cells and left overnight to incubate at 37C.
+
-
</p>
+
-
17th July - Very little colony growth was observed from W3110 E.coli streaked plates. Plates were therefore left to incubate for a further 17 hours.
+
-
</p>
+
-
18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media [link to LB recipe] + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
+
-
</p>
+
-
19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
+
-
</p>
+
-
Mammalian Lab
+
-
</p>
+
-
17th July - Mammalian cell culture and maintenance [link to mammalian protocol] training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
+
-
</p>
+
-
18th July - MEF passage 4
+
-
</p>
+
-
19th July - MEF passage 5
+
-
</p>
+
-
</p>
+
-
 
+
-
<p class="minor_title">Week 8</p>
+
-
<p class="body_text">
+
-
Bacterial Lab
+
-
</p>
+
-
22nd July -Transformation [link to transformation protocol] of our competent cells with plasmid YB3110 was carried out.
+
-
</p>
+
-
23rd July - No colony growth was observed on Ampicillin plates [link to ampicillin plate protocol] indicating no plasmid uptake. Transformation was repeated with YB3110.
+
-
</p>
+
-
<p class="minor_title">Week 9</p>
+
-
<p class="body_text">
+
-
Bacterial Lab
+
-
<p class="major_title">August</p>
+

Latest revision as of 00:25, 5 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18

Click the links above to view the corresponding Lab diary weeks

Retrieved from "http://2013.igem.org/Team:UCL/Labbook"