Team:TU Darmstadt/result

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<img alt="Problem" src="/wiki/images/6/66/Darmstadt_green_Problem.jpg" width="100" height="30"></a>
<img alt="Problem" src="/wiki/images/6/66/Darmstadt_green_Problem.jpg" width="100" height="30"></a>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/solution">
 
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<img alt="solution" src="/wiki/images/8/87/Darmstadt_green_Solution.jpg" width="100" height="30"></a>
 
<a href="https://2013.igem.org/Team:TU_Darmstadt/result">
<a href="https://2013.igem.org/Team:TU_Darmstadt/result">
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<img alt="result" src="/wiki/images/2/2c/Darmstadt_green_Result.jpg" width="80" height="30"></a>
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<img alt="result" src="/wiki/images/c/ca/04._Result_(angewählt).jpg" width="80" height="30"></a>
<a href="https://2013.igem.org/Team:TU_Darmstadt/safety">
<a href="https://2013.igem.org/Team:TU_Darmstadt/safety">
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<h1 align="center"><font size="6" color="#F0F8FF" face="Arial regular">Our results</font></h1>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/result/molecular_engineering">
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<font size="8" color="#F0F8FF" face="Arial regular">Molecular engineering |</font>
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</a>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/result/electrical_engineering">
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<font size="8" color="#F0F8FF" face="Arial regular">Electrical engineering </font>
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<b>LSSmOrange:</b><br>
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<img alt="Eppis" src="https://static.igem.org/mediawiki/2013/2/2f/Eppis.JPG" width="400" hight="500" align="left">
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LSSmOrange is an orange fluorescent protein that we want to use for FRET, we us it as a donor. In an
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actual paper Daria M. Shcherbakova[1] showed that LSSmOrange has an excitation maximum by
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<img alt="mainboard" src="https://static.igem.org/mediawiki/2013/7/75/Electric_main.JPG" width="400" hight="500" align="right">
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437 nm and an emission maximum by 572 nm.
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In the following diagram (figure 1) one can see our results with our own expressed LSSmOrange. The
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colored dots show the different maximums. The excitation maximum is at 415 nm, another at 453 nm
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and our measured emission maximum is at 564 nm. This aberration changes nothing
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on the FRET system. The excitation maximum is big enough to stimulate mKate, the other fluorescent
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protein. Of course, we checked also the emission and excitation of our Bl21DE3 cells (data
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not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/DetectionConstruct">
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<img alt="Labbookdetection" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif">
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<font size="6" color="#F0F8FF" face="Arial regular">Visit our lab book for the detection construct</font><br />
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</a>
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<br><br>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/Biobricks">
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<img alt="Labbookbiobricks" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif">
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<font size="6" color="#F0F8FF" face="Arial regular">Visit our lab book for the fluorescence proteins</font><br />
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</a>
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<br><br>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/safety/Labjournal">
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<img alt="Labbookbiobricks" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif">
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<font size="6" color="#F0F8FF" face="Arial regular">Visit our lab book for the safety construct</font><br />
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</a>
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<br>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook/Cutinase">
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<img alt="Labbookdetection" border="5" width="57,5" height="50" src="/wiki/images/8/81/Book2.gif">
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<font size="6" color="#F0F8FF" face="Arial regular">Visit our lab book for the cutinase</font><br />
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</a>
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Latest revision as of 03:32, 5 October 2013







Molecular engineering | Electrical engineering




Eppis mainboard














Labbookdetection Visit our lab book for the detection construct

Labbookbiobricks Visit our lab book for the fluorescence proteins

Labbookbiobricks Visit our lab book for the safety construct

Labbookdetection Visit our lab book for the cutinase

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/result"