Team:Tsinghua-E/Project

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<li><a href="https://2013.igem.org/Team:Tsinghua-E/Project">Overview</a></li>
<li><a href="https://2013.igem.org/Team:Tsinghua-E/Project">Overview</a></li>
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         <li><a href="https://2013.igem.org/Team:Tsinghua-E/Project">Results</a></li>
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         <li><a href="https://2013.igem.org/Team:Tsinghua-E/Result">Results</a></li>
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== '''Overall project''' ==
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<p>  The major idea of our team is to simulate the process of evolution by involving e.coil groups producing tryptophan.</p>
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<p>  As our research object, e.coil’s propagation offers the possible condition of heredity.In order to accelerate as well as regulate its mutation frequency, we expect to introduceinto e.coi an Artificial mutation machinery --- mutD.</p>
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<p>　As to surviving pressure, we have designed a gene circuit responding to the level of tryptophan. We knockout the original maltose hydrolysis gene (malQ) of e.coli and add a new malQ gene together with the mutD gene following a tryptophan sensor. Then we transfer the plasmid to the previous e.coli JW3379. Thus, the higher the level of tryptophan turns, the more maltose hydrolysis gene will be transcribed and the less mutD gene. In the simulation of evolution, we culture the e. coli in the M9 media containing maltose as the only carbon source, and provide a tryptophan doze at the beginning of the cultivation and gradually reduce the level of tryptophan in the media. Providing all the treatment mentioned above, we expect that only the mutation producing high level of tryptophan will produce enough maltose to survive the media. When tryptophan reaches a critical level, the expression of mutD will be totally depressed and mutation ceases, leaving the evolved as well as the desired e.coli in the media.</p>
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<p> Thanks for Natural Selection, we believe that the method of simulation of evolution used in our project will become a global approach to produce any desired chemicals using e.coli in the near future!</p>
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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<h2>Overview</h2>
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<h3 align="center"><strong>Darwinian evolution for microbial cell factory:</strong><br />
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<em>in vivo</em> evolution engineering towards tryptophan-overproduction superbug</h3>
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Darwinian evolution shows great power in creating incredible biological function in amazing speed.Inspired by this, our team aimed at creating novel fast and irrational microbial cell factory by simulating natural Darwinian evolution process. With tryptophan as target product, a novel tryptophan biosensor utilizing translating ribosome mechanism was firstly developed as the foundation for tryptophan productivity and selection pressure switch module. We further constructed this tryptophan overproduction selection gene circuit coupling with in vivo mutation machine (mutator gene of mutD). By fine-tuning the selection conditions, our selection circuit showed good tryptophan dependent growth property, which provides the foundation for further evolution.As a preliminary result of this project, we successfully evolved an ancestor with zero productivity to a high-tryptophan producer only after several rounds of evolution.
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Latest revision as of 09:53, 10 October 2013

  • Totop

















Overview

Darwinian evolution for microbial cell factory:
in vivo evolution engineering towards tryptophan-overproduction superbug

Darwinian evolution shows great power in creating incredible biological function in amazing speed.Inspired by this, our team aimed at creating novel fast and irrational microbial cell factory by simulating natural Darwinian evolution process. With tryptophan as target product, a novel tryptophan biosensor utilizing translating ribosome mechanism was firstly developed as the foundation for tryptophan productivity and selection pressure switch module. We further constructed this tryptophan overproduction selection gene circuit coupling with in vivo mutation machine (mutator gene of mutD). By fine-tuning the selection conditions, our selection circuit showed good tryptophan dependent growth property, which provides the foundation for further evolution.As a preliminary result of this project, we successfully evolved an ancestor with zero productivity to a high-tryptophan producer only after several rounds of evolution.


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